装载ACE2的植物来源细胞外囊泡防治PM_(2.5)暴露后细胞损伤  

Plant-derived extracellular vesicles loaded with ACE2 prevent cell damage after PM_(2.5)exposure

作　　者：叶旋 钤嫄 王红云 周蕾[1] YE Xuan;QIAN Yuan;WANG Hongyun;ZHOU Lei(Department of Cardiology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China;School of Life Science,Shanghai University,Shanghai 200444,China)

机构地区：[1]南京医科大学第一附属医院心内科,南京210029 [2]上海大学生命科学学院,上海200444

出　　处：《上海大学学报（自然科学版）》2024年第2期208-217,共10页Journal of Shanghai University:Natural Science Edition

基　　金：国家自然科学基金面上资助项目(81970723);上海市教育发展基金会和上海市教委“晨光计划”资助项目(20CG46)。

摘　　要：探究装载血管紧张素转化酶2(angiotensin converting enzyme 2,ACE2)的植物来源细胞外囊泡(plant-derived extracellular vesicles,PDEVs)在保护机体抵抗空气污染颗粒物(particulate matter,PM_(2.5))引发的呼吸系统损伤过程中的作用.基于葡萄提取PDEVs并装载ACE2过表达的质粒,通过CCK8实验、二氢乙锭(dihydroethidium,DHE)染色、蛋白免疫印迹、实时荧光定量多聚酶链反应(polymerase chain reaction,PCR)等方法,探究改造后的PDEVs对PM_(2.5)暴露后人支气管上皮细胞(Beas-2B)的保护效果.PM_(2.5)暴露会造成Beas-2B细胞氧化应激、细胞凋亡增加,以及炎症因子增加,而装载ACE2的PDEVs能够有效递送ACE2,从而缓解PM_(2.5)诱导的细胞损伤.因此,PDEVs可作为潜在的递送载体,防治大气PM_(2.5)暴露引发的细胞凋亡及损伤.To investigate the efficacy of plant-derived extracellular vesicles(PDEVs)loaded with angiotensin-converting enzyme 2(ACE2)in mitigating respiratory system damage induced by particulate matter(PM_(2.5)),we extracted PDEVs from grapes and loaded them with ACE2 overexpression plasmids.The effect of the modified PDEVs on human bronchial epithelial cells(Beas-2B)after exposure to PM_(2.5)was investigated using CCK8 assays,dihydroethidium(DHE)staining,western blotting,and real-time quantitative polymerase chain reaction(PCR).Exposure to PM_(2.5)increases oxidative stress,apoptosis,and inflammatory factor production in Beas-2B cells.PDEVs loaded with ACE2 effectively delivered ACE2 to cells and thereby alleviated PM_(2.5)-induced cell damage including apoptosis,oxidative stress,and inflammation.PDEVs thus show potential for use as carriers of genes that can prevent apoptosis and damage caused by atmospheric PM_(2.5)exposure.

关 键 词：细胞外囊泡 血管紧张素转化酶2 PM_(2.5) 葡萄 

分 类 号：R994.6[医药卫生—毒理学]