Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development  

作　　者：Anisa-Annur S Wan-Hafizah WJ Nor-Ashikin MNK Muhammad-Zaki R 

机构地区：[1]Faculty of Pharmacy and Health Sciences,Royal College of Medicine Perak,Universiti Kuala Lumpur,30450 Ipoh,Perak,Malaysia [2]Maternofetal and Embryo Research Group,Faculty of Medicine,Universiti Teknologi MARA,Selangor Branch,Sungai Buloh Campus,47000 Sungai Buloh,Selangor,Malaysia

出　　处：《Asian pacific Journal of Reproduction》2024年第3期126-132,共7页亚太生殖杂志（英文版）

基　　金：supported by the Fundamental Research Grant Scheme(FRGS)[FRGS/1/2020/SKK06/UNIKL/02/1],from the Ministry of Higher Education,Malaysia.

摘　　要：Objective:To investigate the effects of coenzyme Q10(CoQ10)supplementation on post-vitrification embryo development and gross morphology.Methods:Balb/c mouse embryos were cultured in potassium simplex optimised medium(KSOM)with varying CoQ10 concentrations[0(control),20,40,and 60μM].The most effective CoQ10 concentration(40μM)was selected for subsequent post-vitrification morphology study.Embryos were randomly divided into four groups:Group A(non-vitrified without CoQ10),Group B(non-vitrified with CoQ10),Group C(vitrified without CoQ10),and Group D(vitrified with CoQ10),followed by vitrification at the 8-cell stage.Survival rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system,distinguishing normal and abnormal embryos.Results:Supplementation of 40μM CoQ10 significantly increased blastocyst formation(95%)compared to the control group(92%),20μM(62%),and 60μM(56%)(P<0.001).Following vitrification,Group D exhibited a significant increase in blastocyst formation(92%)compared to Group C(82%)(P<0.05).Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage,morula,and blastocyst(P<0.05).Conclusions:CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development,increase blastocyst formation rates,and improve embryo quality post-vitrification.This offers a promising approach to mitigate oxidative stress on embryos,potentially improving overall assisted reproductive technology outcomes.

关 键 词：Coenzyme Q10 ANTIOXIDANT Embryo development VITRIFICATION MORPHOLOGY Assisted reproductive technology 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]