MicroRNA-21/PARP-1作为生物标志物在AR和CARAS中的诊断价值分析  

Analysis of the diagnostic value of microRNA-21/PARP-1 as biomarkers in AR and CARAS

作　　者：肖心儒 丁紫琪 刘志光[1] 毛正道[1] 张倩[1] XIAO Xinru;DING Ziqi;LIU Zhiguang;MAO Zhengdao;ZHANG Qian(Department of Respiratory and Critical Care Medicine,the Affiliated Changzhou NO.2 People’s Hospital of Nanjing Medical University,Changzhou Medical Center of Nanjing Medical University,Changzhou 213003,China)

机构地区：[1]南京医科大学附属常州市第二人民医院呼吸与危重症医学科,南京医科大学常州医学中心,江苏常州213003

出　　处：《南京医科大学学报（自然科学版）》2024年第6期860-867,共8页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省社会发展项目(BE2020651);江苏省“333”高层次人才项目(BRA2020015);常州市高层次医学人才项目(2022CZLJ013);南京医科大学常州医学中心科研项目(CMCB202214)。

摘　　要：目的:评估外周血microRNA(miR)-21、血浆聚腺苷二磷酸核糖聚合酶1[poly(ADP-ribose)polymerase-1,PARP-1]在过敏性鼻炎(allergic rhinitis,AR)和过敏性鼻炎-哮喘综合征(combined allergic rhinitis and asthma syndrome,CARAS)中的诊断价值。方法:收集44例CARAS患者、31例AR患者和42例健康对照的外周血,采用RT-qPCR法检测外周血中miR-21的表达水平,采用ELISA法检测血浆中PARP-1蛋白水平。应用Pearson进行相关性分析。受试者工作特征(receiver operating characteristic,ROC)曲线判断miR-21和PARP-1的诊断灵敏度与特异度。结果:CARAS组患者外周血miR-21的表达较健康对照组升高。AR组患者血浆PARP-1的水平较CARAS组和健康对照组升高。Pearson相关性分析结果显示,外周血miR-21的表达水平在AR患者中与嗜酸性粒细胞计数相关,在CARAS患者中与鼻呼出气一氧化氮(fractional nasal nitric oxide,FnNO)水平相关;血浆PARP-1在AR患者中与1秒钟用力呼气量占预计值百分比(forced expiratory volume in onesecond percent predicted,FEV_(1)%_(pred))相关,在CARAS患者中与FEV_(1)%_(pred)及1秒钟用力呼气量(forced expiratory volume in one second,FEV_(1))/用力肺活量(forced vital capacity,FVC)(FEV_(1)/FVC)相关。ROC曲线分析显示,外周血miR-21作为CARAS的诊断标志物时,灵敏度为51.35%,特异度为80.95%。血浆PARP-1作为AR的诊断标志物时,灵敏度为90.32%,特异度为54.76%。血浆PARP-1作为AR进展为CARAS的诊断标志物时,灵敏度为45.45%,特异度为90.32%。结论:AR和CARAS患者外周血miR-21、PARP-1存在差异表达,外周血miR-21可作为CARAS的诊断标志物,PARP-1可作为AR的诊断标志物及AR进展为CARAS的生物标志物。这对寻求AR和CARAS的诊治靶点有十分重要的价值。Objective:To evaluate the diagnostic value of peripheral blood microRNA(miR)-21 and plasma poly(ADP-ribose)polymerase-1(PARP-1)in allergic rhinitis(AR)and combined allergic rhinitis and asthma syndrome(CARAS).Methods:Peripheral blood samples were collected from 44 CARAS patients,31 AR patients,and 42 healthy controls.The expression levels of miR-21 in peripheral blood were detected by RT-qPCR,and the plasma levels of PARP-1 protein were measured by ELISA.Correlation analysis was performed by rearson correlation analysis.The diagnostic sensitivity and specificity of miR-21 and PARP-1 were determined by receiver operating characteristic(ROC)curve.Results:The expression of peripheral blood miR-21 was high in CARAS patients compared with healthy controls.The level of PARP-1 was higher in AR patients than that in CARAS patients and healthy controls.Pearson correlation analysis showed that the expiression of miR-21 was correlated with eosinophils count in AR patients and with fractional nasal nitric oxide(FnNO)in CARAS patients.The plasma level of PARP-1 was correlated with forced expiratory volume in one second percent predicted(FEV_(1)%_(pred))in CARAS patients and with FEV_(1)%_(pred) and forced expiratory volume in one second(FEV_(1))/forced vital capacity(FVC)(FEV_(1)/FVC)in CARAS patients.ROC curve analysis showed that when peripheral blood miR-21 was used as a diagnostic marker for CARAS,the sensitivity was 51.35%and the specificity was 80.95%.When the plasma PARP-1 was used as a diagnostic marker for AR,the sensitivity was 90.32%and the specificity was 54.76%.When the plasma PARP-1 was used as a diagnostic marker for the progression from AR to CARAS,the sensitivity was 45.45%and the specificity was 90.32%.Conclusion:There are differential expressions of miR-21 and PARP-1 in peripheral blood of patients with AR and CARAS.the peripheral bolld miR-21 can serve as a diagnostic biomarker for CARAS,while the plasma PARP-1 can serve as a diagnostic biomarker for AR and as a biomarker for the progression from AR to CAR

关 键 词：过敏性鼻炎-哮喘综合征 MICRORNA-21 PARP-1 生物标志物 

分 类 号：R562.25[医药卫生—呼吸系统]