25-羟基维生素D_3替代维生素D_3对产蛋后期蛋鸡生产性能、蛋品质和钙磷代谢的影响  

Effects of Substituting 25⁃Hydroxyvitamin D3 for Vitamin D3 on Performance,Egg Quality and Calcium and Phosphorus Metabolism of Laying Hens in Late Laying Period

作　　者：高珊 郑君杰 邱凯 张海军[1] 武书庚[1] GAO Shan;ZHENG Junjie;QIU Kai;ZHANG Haijun;WU Shugeng(Laboratory of Quality&Safety Risk Assessment for Animal Products on Feed Hazards,Key Laboratory of Feed Biotechnology,Ministry of Agriculture and Rural Affairs,Institute of Feed Research,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Beijing Agricultural Products Quality and Safety Center,Beijing 100020,China)

机构地区：[1]中国农业科学院饲料研究所,农业农村部饲料生物技术重点实验室,农业农村部动物产品质量安全饲料源性因子风险评估实验室,北京100081 [2]北京市农产品质量安全中心,北京100020

出　　处：《动物营养学报》2024年第5期2992-3002,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：北京市家禽创新团队(BAIC06-2023);国家蛋鸡产业技术体系(CARS-40);抗炎抗病增效产品功能评估与应用(2022YFC2105005);中国农业科学院农业科技创新工程(ASTIP)。

摘　　要：本试验旨在研究饲粮中添加25-羟基维生素D_(3)(25-OHD_(3))替代维生素D_(3)(VD_(3))对产蛋后期蛋鸡生产性能、蛋品质、钙磷代谢、肠道形态及胫骨质量的影响。试验选取270只55周龄采食正常、体重相近(整齐度>90%)的海兰褐蛋鸡,随机分为3个组,每组6个重复,每个重复15只鸡。对照组饲喂添加4000 IU/kg VD_(3)的基础饲粮,试验1组饲喂添加50μg/kg 25-OHD_(3)和2000 IU/kg VD_(3)的饲粮,试验2组饲喂添加100μg/kg 25-OHD_(3)的饲粮。预试期1周,正试期12周。结果表明:1)与对照组相比,试验1组第5~8周产蛋率显著提高(P<0.05),试验1组和试验2组第9~12周产蛋率显著提高(P<0.05)。2)与对照组相比,试验1组和试验2组第4周和第12周以及试验1组第8周蛋壳强度显著提高(P<0.05);试验1组第8周和试验2组第12周蛋白高度显著提高(P<0.05);试验1组和试验2组试验各阶段哈氏单位均显著提高(P<0.05)。3)与对照组相比,试验1组和试验2组血清钙和磷含量以及胫骨钙含量均显著提高(P<0.05)。4)与对照组相比,试验1组和试验2组血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性以及清除超氧阴离子能力和清除羟自由基能力显著提高(P<0.05),血清丙二醛(MDA)含量显著降低(P<0.05);试验1组血清总抗氧化能力(T-AOC)显著提高(P<0.05)。与对照组相比,试验1组血清碳酸酐酶(CA)和甲状旁腺素(PTH)含量显著提高(P<0.05),血清二胺氧化酶(DAO)含量显著降低(P<0.05);试验1组和试验2组血清骨钙素(BGP)含量显著降低(P<0.05);试验2组血清降钙素(CT)含量显著降低(P<0.05)。5)与对照组相比,试验1组空肠绒毛高度显著提高(P<0.05),试验2组空肠隐窝深度显著降低(P<0.05),试验1组和试验2组空肠绒毛高度/隐窝深度值均显著提高(P<0.05)。6)与对照组相比,试验2组胫骨强度、骨小梁数量、骨小梁面积显著提高(P<0.05),试验1组胫骨粗灰分含量显著提高(P<This experiment was conducted to investigate the effects of dietary 25⁃hydroxyvitamin D_(3)(25⁃OHD_(3))as a substitute for vitamin D_(3)(VD_(3))on performance,egg quality,calcium and phosphorus me⁃tabolism,intestinal morphology and tibia quality of laying hens in late laying period.A total of 270 Hy⁃Line brown laying hens aged at 55 weeks with normal feeding and similar body weight(uniformity>90%)were randomly divided into 3 groups with 6 replicates per group and 15 hens per replicate.The hens in the control group were fed a basal diet supplemented with 4000 IU/kg VD_(3),the hens in experimental group 1 were fed a diet supplemented with 50μg/kg 25⁃OHD_(3) and 2000 IU/kg VD_(3),and the hens in experimental group 2 were fed a diet supplemented with 100μg/kg 25⁃OHD_(3).The pre⁃trial period was 1 week and the experimental period was 12 weeks.The results showed as follows:1)compared with the control group,the laying rate in exper⁃imental group 1 in weeks 5 to 8 was significantly increased(P<0.05),and the laying rate in experimental groups 1 and 2 in weeks 9 to 12 was significantly increased(P<0.05).2)Compared with the control group,the eggshell strength in experimental groups 1 and 2 at weeks 4 and 12 and in experimental group 1 at weeks 8 was significantly increased(P<0.05);the albumen height in experimental group 1 at week 8 and in experi⁃mental group 2 at week 12 was significantly increased(P<0.05);the Haugh unit in experimental groups 1 and 2 at each stage was significantly increased(P<0.05).3)Compared with the control group,the contents of calcium and phosphorus in serum and calcium content in tibia in experimental groups 1 and 2 were significantly increased(P<0.05).4)Compared with the control group,the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH⁃Px)and catalase(CAT)as well as the superoxide anion scavenging ability and hydroxyl radical scavenging ability in serum in experimental groups 1 and 2 were significantly increased(P<0.05),and the malondialdehyde(MDA)content in

关 键 词：25-羟基维生素D_3 产蛋后期蛋鸡 生产性能 蛋品质 钙磷代谢 胫骨质量 

分 类 号：S816[农业科学—饲料科学]