硝酸异山梨酯对奶牛体外模拟瘤胃发酵甲烷产量、瘤胃发酵参数和营养物质降解率的影响  

Effects of Isosorbide Nitrate on Methane Production,Rumen Fermentation Parameters and Nutrient Degradation Rates of Dairy Cows in Vitro Simulated Rumen Fermentation

作　　者：付帅旗 杜兴杰 姚朝辉 王世伟 张岩[1] 虎业浩 高腾云[1] FU Shuaiqi;DU Xingjie;YAO Zhaohui;WANG Shiwei;ZHANG Yan;HU Yehao;GAO Tengyun(Henan International Joint Laboratory of Nutrition Regulation and Ecological Raising of Domestic Animal,College of Animal Science and Technology,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学动物科技学院,河南省家畜营养调控与生态养殖国际联合实验室,郑州450002

出　　处：《动物营养学报》2024年第5期3061-3069,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：财政部和农业农村部-国家现代农业产业技术体系资助(CARS-36)。

摘　　要：本试验旨在研究不同水平硝酸异山梨酯(ISDN)对奶牛体外模拟瘤胃发酵甲烷产量、瘤胃发酵参数和营养物质降解率的影响。采用单因素完全随机试验设计,设置2个平行批次,分为5组,每组5个重复。各组底物中ISDN添加水平分别为0(A组,作为对照组)、0.1%(B组)、0.2%(C组)、0.4%(D组)、0.6%(E组)。试验所用底物为全混合日粮(TMR),瘤胃液供体为5头体况相近的成年健康荷斯坦公牛。发酵24和48 h时,测量发酵甲烷产量、瘤胃发酵参数和营养物质降解率。结果表明:1)发酵24和48 h时,D组、E组的产气量、甲烷产量和甲烷比例均显著低于A组(P<0.05)。2)发酵24 h时,D组、E组的瘤胃pH显著低于A组(P<0.05)。发酵24和48 h时,各组之间瘤胃总挥发性脂肪酸(TVFA)浓度无显著差异(P>0.05),但D组和E组的瘤胃乙酸比例、乙酸/丙酸显著低于A组(P<0.05),瘤胃丙酸比例显著高于A组(P<0.05)。3)发酵24 h时,E组的干物质(DM)、中性洗涤纤维(NDF)和酸性洗涤纤维(ANF)降解率均显著低于A组(P<0.05),D组的NDF降解率也显著低于A组(P<0.05);发酵48 h时,各组之间营养物质降解率无显著差异(P>0.05)。综上所述,添加0.4%的ISDN可以有效降低体外模拟瘤胃发酵过程中甲烷产量,且对于营养物质降解率影响较小。The aim of this experiment was to investigate the effects of different levels of isosorbide dinitrate(ISDN)on methane production,rumen fermentation parameters and nutrient degradation rates of dairy cows in vitro simulated rumen fermentation.A one⁃way completely randomized experimental design was used,set up 2 parallel batches,and divided into 5 groups with 5 replicates per group.The ISDN supplemental levels in sub⁃strates of each group were 0(group A,as control group),0.1%(group B),0.2%(group C),0.4%(group D)and 0.6%(group E),respectively.The substrate in the experiment was total mixed ration(TMR),and the rumen fluid donors were five adult healthy Holstein bulls with similar body condition.At 24 and 48 h of fermentation,the methane production,rumen fermentation parameters and nutrient degradation rates were measured.The results showed as follows:1)at 24 and 48 h of fermentation,the gas production methane production and methane proportion of group D and group E were significantly lower than those of group A(P<0.05).2)At 24 h of fermentation,the rumen pH of group D and group E was significantly low⁃er than that of group A(P<0.05).At 24 and 48 h of fermentation,there was no significant difference in ru⁃men total volatile fatty acid(TVFA)concentration among all groups(P>0.05),but the rumen acetic acid proportion and acetic acid/propionic acid of group D and group E were significantly lower than those of group A(P<0.05),and the rumen propionic acid proportion was significantly higher than that of group A(P<0.05).3)At 24 h of fermentation,the dry matter(DM),neutral detergent fiber(NDF)and acid detergent fiber(ANF)degradation rates of group E were significantly higher than that of group A(P<0.05),and the NDF degradation rate of group D was significantly lower than that of group A(P<0.05);at 48 h of fermenta⁃tion,there were no significant differences in nutrient degradation rates among all groups(P>0.05).In summa⁃ry,adding 0.4%ISDN can effectively reduce the methane production during in vitro simulated rumen

关 键 词：体外模拟瘤胃发酵 甲烷 硝酸异山梨酯 瘤胃发酵参数 奶牛 

分 类 号：S823[农业科学—畜牧学]