白藜芦醇对玉米赤霉烯酮所致雌性大鼠子宫和卵巢损伤的影响  

Effects of Resveratrol on Uterine and Ovarian Injury of Zearalenone⁃Induced Female Rats

作　　者：姚丹 李怡娜 成晓丽 何曾文 汪玥 马小军[1] 张勇[1,2] YAO Dan;LI Yina;CHENG Xiaoli;HE Zengwen;WANG Yue;MA Xiaojun;ZHANG Yong(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Gansu Provincial Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省动物生殖生理及繁殖调控重点实验室,兰州730070

出　　处：《动物营养学报》2024年第5期3317-3328,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划子课题——牛羊肉中主要化学性危害物非靶向筛选和精准识别技术应用示范(2019YFC1605705);甘肃省动物生殖生理及繁殖调控重点实验室(20JR10RA563)。

摘　　要：本试验通过建立有效的玉米赤霉烯酮所致雌性大鼠毒性模型,探讨白藜芦醇对玉米赤霉烯酮所致雌性大鼠子宫和卵巢损伤的影响。1)将32只2月龄雌性大鼠随机分为4组,每组8个重复,每个重复1只。对照组大鼠每天腹腔注射玉米油,低、中、高浓度组大鼠分别每天腹腔注射1.5、3.0和5.0 mg/kg玉米赤霉烯酮,每天注射量均为0.5 mL,试验期5 d。2)将32只2月龄雌性大鼠随机分为4组,每组8个重复,每个重复1只。第1~21天,RSV组和RSV+ZEA组大鼠每天灌胃40 mg/kg白藜芦醇,空白组和ZEA组大鼠每天灌胃羧甲基纤维素钠溶液,每天灌胃量均为1 mL;第22~26天,ZEA组和RSV+ZEA组大鼠按上述方法构建玉米赤霉烯酮所致雌性大鼠生殖系统凋亡的动物模型,空白组和RSV组大鼠腹腔注射玉米油。结果表明:1)与对照组相比,中、高浓度组子宫内膜变薄,子宫腺增多,闭锁卵泡数量增多,黄体增大且充血严重,子宫和卵巢中B淋巴细胞瘤-2相关X蛋白(Bax)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的mRNA和蛋白相对表达量显著升高(P<0.05),B淋巴细胞瘤-2(Bcl-2)的mRNA和蛋白相对表达量显著降低(P<0.05)。2)与ZEA组相比,RSV+ZEA组子宫内膜变厚,上皮细胞排列整齐,子宫腺减少;卵巢中可见发育卵泡,闭锁卵泡数量减少,黄体充血减少。与空白组和RSV+ZEA组相比,ZEA组的子宫和卵巢中Bax和Caspase-3的阳性表达增强,而Bcl-2的阳性表达减弱。与ZEA组相比,RSV+ZEA组的子宫和卵巢中Bax和Caspase-3的mRNA和蛋白相对表达量显著降低(P<0.05),Bcl-2的mRNA和蛋白相对表达量显著升高(P<0.05)。由此可见,2月龄雌性大鼠连续腹腔注射玉米赤霉烯酮5 d(每天3.0 mg/kg,玉米油为溶剂)可成功建立雌性大鼠玉米赤霉烯酮毒性动物模型。玉米赤霉烯酮对雌性大鼠子宫和卵巢有损伤,白藜芦醇对玉米赤霉烯酮引起的生殖毒性有明显保护作用。This experiment was conducted to investigate the effects of resveratrol on uterine and ovarian injury of zearalenone⁃induced female rats by establishing an effective toxicity model of zearalenone⁃induced female rats.1)Thirty⁃two 2⁃month⁃old female rats were randomly divided into 4 groups with 8 replicates per group and 1 rat per replicate.Rats in the control group were injected intraperitoneally with corn oil per day,and rats in the low,medium and high concentration groups were injected intraperitoneally with 1.5,3.0 and 5.0 mg/kg of zearalenone per day,respectively,at a daily dose of 0.5 mL for a period of 5 days.2)Thirty⁃two 2⁃month⁃old female rats were randomly divided into 4 groups with 8 replicates per group and 1 rat per replicate.During days 1 to 21,rats in the RSV group and RSV+ZEA group were given with 40 mg/kg resveratrol per day,and rats in the blank group and ZEA group were given with sodium carboxymethylcellulose solution per day,at a volume of 1 mL per day;during days 22 to 26,the animal models of zearalenone⁃induced apoptosis in the re⁃productive system of female rats was constructed for the female rats in ZEA group and RSV+ZEA group ac⁃cording to the above method,and the blank group and RSV group were injected intraperitoneally with corn oil.The results showed as follows:1)compared with the control group,the medium and high concentration groups showed thinner endometrium,more uterine glands,increased of atretic follicles number,enlarged and severely congested corpus luteum,the relative expression levels of B lymphoblastoma⁃2⁃related X protein(Bax)and cysteine aspartate protease⁃3(Caspase⁃3)mRNA and protein in uterus and ovary were significantly increased(P<0.05),and the relative expression levels of B lymphoblastoma⁃2(Bcl⁃2)mRNA and protein were signifi⁃cantly decreased(P<0.05).2)Compared with ZEA group,the RSV+ZEA group had a thickened endometri⁃um with aligned epithelial cells and fewer uterine glands;developing follicles were seen in the ovary,the num⁃ber

关 键 词：玉米赤霉烯酮 白藜芦醇 凋亡 生殖 

分 类 号：S816.7[农业科学—饲料科学]