苹果砧木CX5组织培养体系的建立  

Establishment of CX5 tissue culture system of apple rootstock

作　　者：严家帝 杨安 田玉凤 韩晓蕾[1] 张彩霞[1] YAN Jiadi;YANG An;TIAN Yufeng;HAN Xiaoei;ZHANG Caixia(Institute of Pomology of CAAS,Xingcheng,Liaoning 125100)

机构地区：[1]中国农业科学院果树研究所,辽宁兴城125100

出　　处：《中国果树》2024年第5期39-44,共6页China Fruits

基　　金：国家重点研发计划项目(2022YFD160050);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-27)。

摘　　要：以苹果半矮化砧CX5的休眠枝条茎段和4月底至6月初的新生枝条茎段为试验材料,采用离体组织培养方法,研究了适宜CX5组培快繁的外植体类型、灭菌方式、灭菌时间,以及不同植物生长调节剂浓度对增殖培养的影响。结果表明:适合用作灭菌的CX5外植体类型为新生枝条茎段,其外植体褐化率和污染率都相对较低;外植体0.1%HgCl2灭菌时间控制在6min左右,伤害最小;CX5茎段腋芽诱导培养基为MS+0.75mg/L6-BA+0.3mg/LIBA,适宜组培苗增殖继代的培养基为MS+0.7mg/L6-BA+0.5mg/LIBA,生根培养基为1/2MS+0.2mg/LIAA。初步构建了CX5组培快繁技术体系。Stem segments of dormant branches and stem segments of new branches from late April to early June of apple semi-dwarfing anvils were used as experimental materials.In vitro tissue culture method was used to study the types of explants suitable for rapid propagation of CX5 tissue culture,sterilization method,sterilization time,and the effects of different plant growth regulators on proliferation and culture.The results showed that CX5 explants suitable for sterilization were stem segments of new shoots,and their browning and contamination rates were relatively low.The sterilization time of 0.1%HgCl2 for explants was controlled to about 6 min,and the damage was minimal.CX5 stem axillary bud induction medium was MS+0.75 mg/L 6-BA+0.3 mg/L IBA,suitable for tissue culture seedlings proliferation and subculture medium was MS+0.7 mg/L 6-BA+0.5 mg/L IBA,rooting medium was 1/2 MS+0.2 mg/L IAA.The CX5 tissue culture and rapid propagation technology system was established.

关 键 词：苹果半矮化砧 CX5 茎段 组织培养 

分 类 号：S661.1[农业科学—果树学]