静原鸡miR-2954的靶基因预测及生物信息学分析  

Prediction and bioinformatics analysis of target genes of miR-2954 in Jingyuan chickens

作　　者：杨嘉翊 何亮宏 禹保军 王浩睿 魏榕 付希 胡佳欢 毛向媛 张娟[1] YANG Jiayi;HE Lianghong;YU Baojun;WANG Haorui;WEI Rong;FU Xi;HU Jiahuan;MAO Xiangyuan;ZHANG Juan(College of Agriculture,Ningxia University,Yinchuan 750021,China;Pengyang County Animal Husbandry Technology Extension Service Center,Guyuan 756599,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]彭阳县畜牧技术推广中心,宁夏固原756599

出　　处：《黑龙江畜牧兽医》2024年第9期48-52,118,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：宁夏回族自治区重点研发计划项目“宁夏静原鸡种质资源发掘及产业化配套关键技术研究与示范”(2022BBF02034);宁夏回族自治区财政支农项目“种畜禽(静原鸡)生产性能测定”。

摘　　要：为了预测静原鸡miR-2954的靶基因,并分析miR-2954在静原鸡不同组织的表达差异,探索其在静原鸡肌肉组织生长发育中的调控机制,试验采用生物信息学方法对比miR-2954成熟序列,并分析其保守性,使用TargetScan、miRDB、miRmap在线工具预测其靶基因,对靶基因集合进行GO功能注释和KEGG信号通路富集分析,并采用实时荧光定量PCR方法对miR-2954的组织表达水平进行研究。结果表明:miR-2954序列在鸟类间高度保守,靶基因GO功能条目主要集中在RNA聚合酶Ⅱ对转录的正向调节、信号转导、RNA聚合酶Ⅱ对转录的负向调节、氧化-还原过程、免疫反应和G-蛋白偶联受体信号转导途径等,存在于细胞核、胞浆、外泌体等多个组分中,在ATP结合、锌离子结合、金属离子结合、蛋白质结合等分子功能中也发挥作用。靶基因主要富集于细胞因子-细胞因子受体的相互作用、肌动蛋白细胞骨架的调节、细胞黏附分子、AGE-RAGE信号通路在糖尿病并发症中的作用、胰岛素信号传递途径与脂肪细胞因子信号传递途径等信号通路。miR-2954在静原鸡公鸡肺脏、肝脏中的相对表达量极显著高于其他组织(P<0.01),在静原鸡母鸡肺脏、肾脏、胸肌、腿肌中的相对表达量极显著高于其他器官(P<0.01)。说明miR-2954可通过调节其靶向基因影响静原鸡的生长发育。In order to predict the target genes of miR-2954 in Jingyuan chickens,analyze the expression differences of miR-2954 in different tissues of Jingyuan chickens,and to explore its regulatory mechanism in the growth and development of muscle tissues in Jingyuan chickens,bioinformatics methods were used to compare the mature sequences of miR-2954,and analyze its conserved properties.TargetScan,miRDB,miRmap online tools were used to predict the target genes,and then the target gene set was analyzed by GO functional annotation and KEGG signaling pathway enrichment,and the tissue expression level of miR-2954 was studied by real-time fluorescence quantitative PCR.The results showed that the miR-2954 sequence was highly conserved among birds,and the GO functional entries of the target genes mainly focused on the positive regulation of transcription by RNA polymerase Ⅱ,signal transduction,negative regulation of transcription by RNA polymerase Ⅱ,oxidation-reduction process,immune response,and G-protein coupled receptor signaling pathway.It existed in multiple components such as nucleus,cytoplasm,and exosomes,and also plays a role in molecular functions such as ATP binding,zinc ion binding,metal ion binding,protein binding and other molecular functions.The target genes were mainly enriched in signaling pathways such as cytokine-cytokine receptor interactions,regulation of actin cytoskeleton,cell adhesion molecules,AGE-RAGE signalling pathway in diabetic complications,insulin signalling pathway and adipocytokine signalling pathway and other signalling pathways.The relative expression level of miR-2954 in the lung and livers tissues of Jingyuan chickens was significantly higher than that in other tissues(P<0.01),and the relative expression of miR-2954 in lung,kidney,pectoral muscle and leg muscles of Jingyuan chickens was significantly higher than that in other organs(P<0.01).The results indicated that miR-2954 could affect the growth and development of Jingyuan chickens by regulating its target genes.

关 键 词：静原鸡 miR-2954 MIRNA 靶基因 生物信息学 

分 类 号：S813.3[农业科学—畜牧学]