微小膜壳绦虫感染仓鼠小肠组织病理学观察及MUC2表达的分析  

Histopathological observation and analysis of MUC2 expression in the small intestine of hamsters infected with Hymenolepis nana

作　　者：王鸿雁 李小毛 崔轩胤 张科 牟荣[1,2] WANG Hongyan;LI Xiaomao;CUI Xuanyin;ZHANG Ke;MOU Rong(Department of Parasitology,School of Basic Medicine,Guizhou Medical University,Guiyang 561113,China;Characeristic and Key Laboratory of Modern Pathogenic Biology,Guizhou Medical University,Guiyang 561113,China;The People’s Hospital of Tongren City,Clinical laboratory,Tongren 554300,China)

机构地区：[1]贵州医科大学基础医学院人体寄生虫学教研室,贵阳561113 [2]贵州医科大学现代病原生物学特色重点实验室,贵阳561113 [3]铜仁市人民医院检验科,贵州铜仁554300

出　　处：《黑龙江畜牧兽医》2024年第9期109-113,121,122,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(82160398);贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2021]153)。

摘　　要：为了解微小膜壳绦虫感染仓鼠小肠组织的病理学特征及黏蛋白2(MUC2蛋白)的表达情况,试验从贵阳市宠物市场随机选取60只宠物仓鼠,采用饱和盐水浮聚法检测仓鼠粪便中的虫卵并进行鉴定,根据仓鼠感染微小膜壳绦虫的情况将仓鼠分为未感染组、无症状感染组、普通型感染组和重型感染组,解剖所有仓鼠获取肠道内寄生虫及小肠组织,制备小肠组织切片并进行苏木精-伊红(H.E.)染色、阿利新蓝-过碘酸-雪夫(AB-PAS)染色及免疫组织化学染色,通过实时荧光定量PCR(qPCR)扩增分析仓鼠小肠MUC2基因相对表达情况。结果表明:微小膜壳绦虫在宠物仓鼠中的感染率为74.5%,其感染可引起仓鼠小肠绒毛损伤、上皮细胞变性坏死、杯状细胞异常、白细胞浸润等病理改变;与未感染组相比,无症状组杯状细胞数量及MUC2基因相对表达量差异不显著(P>0.05),普通型感染组的杯状细胞数量极显著增加(P<0.01),重型感染组的杯状细胞极显著减少(P<0.01),同时重型感染组的MUC2基因及MUC2蛋白表达水平极显著增高(P<0.01)。说明微小膜壳绦虫感染会导致仓鼠小肠绒毛结构破坏,杯状细胞数量及形态异常,并引起杯状细胞分泌MUC2蛋白增多,且重型感染组杯状细胞数量减少,但其分泌的MUC2蛋白量增加。This study investigates the pathological characteristics of Hymenolepis nana infection in hamster small intestine tissues and the expression of Mucin 2(MUC2).A total of 60 pet hamsters were randomly selected from the Guiyang pet market.Using a saturated salt flotation method,the hamster feces were examined for parasite eggs,and based on the degree of Hymenolepis nana infection,hamsters were categorized into uninfected,asymptomatic,mild infection,and severe infection groups.Hamsters were dissected to collect both the intestinal parasites and the small intestinal tissues.Subsequently,the tissues were sectioned and subjected to staining techniques such as Hematoxylin-Eosin(H.E.),Alcian Blue-Periodic Acid-Schiff(AB-PAS),and immunohistochemistry.Real-time quantitative PCR(qPCR) was employed to analyze the relative expression of the MUC2 gene in the hamster small intestine.The results revealed a 74.5% infection rate of Hymenolepis nana in hamsters,which led to pathological alterations including damage to the hamster's small intestinal villi,degeneration and necrosis of epithelial cells,abnormal goblet cells,and infiltration of leukocyte.In comparison to the uninfected group,the asymptomatic group showed no significant differences in goblet cell count or relative expression levels of the MUC2 gene(P > 0.05).However,the mild infection group exhibited a highly significant increase in goblet cell count(P<0.01),while the severe infection group showed a highly significant decrease(P<0.01).Simultaneously,the severe infection group demonstrated a highly significant increase in MUC2 mRNA and MUC2 protein expression levels(P<0.01).This indicatesd that Hymenolepis nana infection resultsed in the disruption of the hamster′s small intestinal villi structure,abnormal goblet cell count and morphology,leading to heightened secretion of MUC2 protein.Furthermore,the decrease in goblet cells in the severe infection group are inversely related to the secretion of MUC2 protein.

关 键 词：仓鼠 微小膜壳绦虫 小肠 病理学特征 黏蛋白2 

分 类 号：R383.19[医药卫生—医学寄生虫学]