赤芝提取物的抗氧化美白及细胞增殖作用研究  

Antioxidative and Whitening Ability and Effect on Cell Proliferation of Ganoderma lucidum Extract

作　　者：木开代斯·买合木提 陈建 焦春伟 谢意珍 MuKaiDaiSi·MaiHeMuTi;CHEN Jian;JIAO Chunwei;XIE Yizhen(College of Resources and Environment,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Yuewei Edible Fungi Technology Co.,LTD,Guangzhou 510663,China;Guangdong Yuewei Biotechnology Co.,LTD,Guangzhou 526238,China)

机构地区：[1]仲恺农业工程学院资源与环境学院,广州510225 [2]广东粤微食用菌技术有限公司,广州510663 [3]广东粤微生物科技有限公司,广州526238

出　　处：《世界中医药》2024年第7期949-956,共8页World Chinese Medicine

基　　金：国家重点研发计划项目子课题(2019YFC1606304-02b);2021年广州市科技计划项目[(援藏)202103000074];2020年广东省国际合作项目(2020A0505100021);广州开发区创新领军人才项目(2020-l010);肇庆高新区创新团队项目(2019ZHQ10)。

摘　　要：目的:研究赤芝提取物(GLE)抗氧化美白及细胞增殖作用。方法:以测定GLE中糖类含量,体外1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除力、蘑菇酪氨酸酶活性抑制力、抑制B16F10细胞黑色素合成及酪氨酸酶活性抑制、结晶紫实验和划痕实验为评价指标,研究GLE抗氧化美白及对L929成纤维细胞增殖的作用。结果:GLE中富含总糖、多糖,GLE相较于β-熊果苷对DPPH自由基和ABTS自由基表现出较弱的清除作用,其自由基清除率半抑制浓度(IC 50)分别为1.6 mg/mL和1.2 mg/mL;对蘑菇酪氨酸酶活性有很强的抑制能力,基于α-MSH促黑激素细胞表型造模实验显示,0.4 mg/mL和0.8 mg/mL的GLE具有显著抑制B16F10细胞酪氨酸酶活性以及黑色素生成的作用;GLE具有促进L929成纤维细胞增殖作用。结论:GLE是美白及功能食品开发利用领域的候选者。Objective:To study the antioxidative and whitening ability of Ganoderma lucidum extract(GLE)and its effects on cell proliferation.Methods:The content of carbohydrate in GLE was measured and GLE′s ability to scavenge in vitro DPPH and ABTS free radicals,inhibit the activity of mushroom tyrosinase,and inhibit melanin synthesis and tyrosinase activity in B16F10 cells,crystal test,and scratch test were set as evaluation indexes to study the antioxidative and whitening ability of GLE and its effects on the proliferation of L929 fibroblasts.Results:GLE was rich in total sugars and polysaccharides.Compared withβ-arbutin,GLE showed a weaker scavenging effect on DPPH free radicals and ABTS free radicals,with IC50 of the free radical scavenging rate being 1.6 mg/mL and 1.2 mg/mL,respectively.It showed a strong inhibitory effect on the activity of mushroom tyrosinase.The results of the phenotype modeling experiment based onα-MSH cells showed that 0.4 mg/mL and 0.8 mg/mL GLE significantly inhibited the activity of tyrosinase and the production of melanin in B16F10 cells.GLE could promote the proliferation of L929 fibroblasts.Conclusion:GLE has great potential for the development and utilization of whitening and functional food.

关 键 词：赤芝提取物 酪氨酸酶活性 B16F10细胞 黑色素 自由基 抗氧化 增殖 L929成纤维细胞 

分 类 号：R285.5[医药卫生—中药学]