五倍瘢痕膏与硅酮霜治疗兔模型增生性瘢痕的对比研究  

A comparative study on treating hypertrophic scar in rabbit model with quintuple scar cream and silicone cream

作　　者：涂亚澜 杨春艳 杨文信 TU Yalan

机构地区：[1]西南医科大学附属中医医院,四川泸州646000

出　　处：《中医临床研究》2024年第11期138-143,共6页Clinical Journal Of Chinese Medicine

基　　金：四川省中医药管理局基金资助项目(2022CP7325);全国名老中医药专家传承工作室项目(【2022】75号);四川省名老中医专家工作室项目(【2021】12号)。

摘　　要：目的:探究五倍瘢痕膏与硅酮霜治疗增生性瘢痕的对比研究。方法:选择27只健康清洁新西兰大耳白兔,根据长轴走形避开大血管在腹侧建立增生性瘢痕模型,每只兔耳朵建立6个圆形创面,共324个创面。按照随机数字表法将这27只新西兰大耳白兔分为对照组、五倍瘢痕膏组(前期经25%、50%及75%五倍瘢痕膏浓度验证,最后取50%五倍瘢痕膏)、硅酮霜组,各9只。术毕消毒,28d后,当创面完全上皮化,将等量膏剂均匀涂抹在瘢痕面,每天2次,治疗28d。比较三组瘢痕增生指数、成纤维细胞数密度、胶原纤维面密度、I型和Ⅲ型胶原蛋白免疫组化与蛋白质免疫印迹检测结果。结果:硅酮霜组瘢痕增生指数、成纤维细胞数密度、胶原纤维面密度明显低于五倍瘢痕膏组和对照组,五倍瘢痕膏组低于对照组,三组差异有统计学意义(P<0.05)。免疫组化结果与蛋白质免疫印迹检测显示硅酮霜组Ⅰ型胶原蛋白相对表达和Ⅲ型胶原蛋白相对表达明显低于五倍瘢痕膏组和对照组,五倍瘢痕膏组低于对照组,三组差异有统计学意义(P<0.05)。结论:五倍瘢痕膏与硅酮霜治疗增生性瘢痕疗效显著,均能显著改善瘢痕组织颜色、减少瘢痕厚度,使其变柔和,降低成纤维细胞数、胶原纤维聚集及增生性瘢痕Ⅰ型胶原蛋白和Ⅲ型胶原蛋白表达,防治瘢痕形成,进而促进康复,但硅酮霜优势更明显。Objective:To explore the comparative study of quintuple scar cream and silicone cream in the treatment of hypertrophic scar.Methods:A total of 27 healthy cleaning New Zealand large ear white rabbits were selected,and a hypertrophic scar model was established on the ventral side according to the long axis to avoid large blood vessels.Six circular wounds were established in each rabbit ear,with a total of 324 wounds.The random number table method was used to divided them into the control group,the quintuple scar cream group(after the verification of the concentration of 25%,50%and 75%quintuple scar cream in the early stage,quintuple scar cream with the concentration of 50%was used finally),and the silicone cream group,with 9 rabbits in each group.After the operation,disinfection was performed.After 28 days,when the wound was completely epithelialized,the same amount of cream was evenly applied to the scar surface,twice a day,for 28 days of treatment.The results of scar hyperplasia index,fibroblast number density,collagen fibrotic surface density,type I and type III collagen protein immunohistochemistry and Western blotting were compared among the three groups.Results:The scar hyperplasia index,fibroblast number density and collagen fiber surface density in the silicone cream group were significantly lower than the quintuple scar cream group and the control group,and the above indicators in the quintuple scar cream group was lower than the control group.The differences among the three groups were significant(P<0.05).Immunohistochemical results and Western blotting detection showed that the relative expressions of type I collagen and type III collagen in the silicone cream group were significantly lower than those in the quintuple scar cream group and the control group,and those in the quintuple scar cream group was lower than the control group,with statistical significant differences among the three groups(P<0.05).Conclusion:Both quintuple scar cream and silicone cream show remarkable effects on hypertrophic scar,bo

关 键 词：增生性瘢痕 五倍瘢痕膏 Ⅰ型胶原 Ⅲ型胶原 硅酮霜 

分 类 号：R619.6[医药卫生—外科学]