脊柱手术后肺部感染患者肠道菌群及肠道屏障功能变化  

The changes of intestinal microbiota and intestinal barrier function in patients with pulmonary infection after spinal surgery

作　　者：胡文静 曹魏莉 HU Wenjing;CAO Weili(Department of Orthopedics,the Second Affiliated Hospital of Air Force Military Medical University,Xi'an,Shaanxi 710038,China;不详)

机构地区：[1]空军军医大学第二附属医院骨科,陕西西安710038

出　　处：《中国微生态学杂志》2024年第4期453-456,461,共5页Chinese Journal of Microecology

摘　　要：目的 研究脊柱手术后肺部感染患者肠道菌群及肠道屏障功能变化,并分析其相关性,为该类患者的治疗提供参考。方法 选取2020年1月至2022年12月于我院行脊柱手术的150例患者为研究对象,根据术后有无并发肺部感染分为感染组(n=53例)与未感染组(n=97例)。检测两组患者肠道菌群紊乱情况与菌群数量;采用酶联免疫吸附法检测血清二胺氧化酶、D-乳酸水平;通过16S rRNA基因测序技术分析肠道菌群相对丰度;采用Person相关性分析肠道菌群、肠屏障功能指标与合并肺部感染的相关性。结果 感染组患者肠道菌群正常22例,未感染组肠道菌群正常76例,感染组肠道菌群紊乱率高于未感染组(58.49%vs 21.65%,P<0.05)。感染组患者肠道乳杆菌、双歧杆菌、拟杆菌数量均低于未感染组,而肠球菌、消化链球菌数量均高于未感染组(均P<0.05)。感染组患者血清二胺氧化酶、D-乳酸水平均高于未感染组(均P<0.05)。150例脊柱手术患者肠道菌群标本中总共发现菌属342个,其中感染组患者肠道消化链球菌、肠球菌相对丰度均高于未感染组,而拟杆菌、双歧杆菌、乳杆菌相对丰度均低于未感染组(均P<0.05)。Person分析显示,乳杆菌、双歧杆菌、拟杆菌数量与术后肺部感染呈负相关,而肠球菌数量、消化链球菌数量、二胺氧化酶、D-乳酸水平与肺部感染呈正相关(均P<0.05)。结论 脊柱术后肺部感染患者肠道菌群紊乱,肠道屏障功能下降,二者与肺部感染的严重程度有关。Objective To explore the changes of intestinal microbiota and intestinal barrier function in patients with pulmonary infection after spinal surgery, and discuss the correlations, providing a reference for the treatment. Methods One hundred and fifty patients who underwent spinal surgery in our hospital from January 2020 to December 2022 were selected, and assigned into infection group (n=53) and non-infection group (n=97) according to the presence or absence of postoperative pulmonary infection. The disturbance of intestinal microbiota and bacterial count were detected, and the serum levels of diamine oxidase and D-lactate were measured using enzyme-linked immunosorbent assay. The relative abundance of gut microbiota was analyzed through 16S rRNA gene sequencing. Pearson correlation analysis was conducted to verify the correlation between gut microbiota, barrier function indicators, and pulmonary infections after spinal surgery. Results There were 22 cases with normal gut microbiota in infection group and 76 cases in non-infection group. The disturbance rate of gut microbiota was 58.49% in infection group, which was higher than the 21.65% in non-infection group (all P<0.05). The counts of Lactobacillus, Bifidobacterium and Bacteroides in infection group were lower, while those of Enterococcus and Peptostreptococcus were higher than those in non-infection group, respectively (all P<0.05). The serum levels of diamine oxidase and D-lactate in infection group were higher than those in non-infection group, respectively (all P<0.05). A total of 342 bacterial genera were found in the microbiota samples of 150 patients undergoing spinal surgery. The relative abundances of Peptostreptococcus and Enterococcus in the infection group were higher, while those of Bacteroides, Bifidobacterium and Lactobacillus were lower than those in the non-infection group, respectively (all P<0.05). Pearson correlation analysis denoted that the counts of Lactobacillus, Bifidobacterium and Bacteroides were negatively correlated with postopera

关 键 词：脊柱手术 肺部感染 肠道菌群 肠道屏障功能 相关性 

分 类 号：R639[医药卫生—外科学]