不同种质基础玉米材料诱导效率及加倍效率研究  

Study on the Induction Efficiency and Doubling Efficiency of Maize with Different Germplasm Bases

作　　者：鲁俊田[1] 吴媛媛 王俊龙 吕春波 丰光[1] 杨海龙[1] 王亮[1] 陈爱国[3] LU Jun-tian;WU Yuan-yuan;WANG Jun-long;LÜChun-bo;FENG Guang;YANG Hai-long;WANG Liang;CHEN Ai-guo(Dandong Academy of Agricultural Sciences,Fengcheng 118109;Liaoning Danyu Seed Science and Technology Co.,Ltd.,Fengcheng 118109;Liaoning Academy of Agricultural Sciences,Shenyan 110136,China)

机构地区：[1]丹东农业科学院,辽宁凤城118109 [2]凤城市农业综合行政执法队,辽宁凤城118109 [3]辽宁省农业科学院,沈阳110136

出　　处：《玉米科学》2024年第2期1-7,共7页Journal of Maize Sciences

基　　金：辽宁省瓦房店市、庄河市、凤城市、义县、大石桥市5个县农作物种质资源系统调查与抢救性收集(19210827)。

摘　　要：以不同类群骨干自交系PH4CV、Mo17、PH6WC、郑58、D299、H29、丹黄34、丹598、昌7-2和S121为基础试材,按照群内和群间杂交共组配15个基础群体,分别形成F_(1)、F_(2)、F_(3)和F_(4)共4个自交世代,研究其单倍体诱导率和加倍率。利用高频诱导系丹诱3号对骨干自交系和不同遗传基础的自交后代进行单倍体诱导,利用浓度为0.06%的秋水仙素对各世代单倍体子粒进行加倍处理。结果表明,不同种质类群自交系间诱导率存在显著差异,以Lancaster群代表系PH4CV、Mo17和Reid群代表系PH6WC、郑58诱导率较高。高诱导率类群通过群内和群间组配基础试材,同样具有较高的诱导率,同时利用杂种优势提升了LRC、SPT和PB群骨干系的诱导率。诱导率随着自交世代增加而显著降低,在F_(2)世代加倍效果最好。There are differences in the induction rate and doubling rate of population constructed by different genetic basis breeding test materials.In order to solve the problem of low induction rate and doubling rate of maize haploid breeding at present,in this study,the backbone inbred lines of different populations PH4CV,Mo17,PH6WC,Zheng 58,D299,H29,Danhuang 34,Dan598,Chang7-2 and S121 were used as the basic materials.According to intra-group and inter-group hybridization,a total of 15 basic populations were assembled,and four inbred generations of F_(1),F_(2),F_(3)and F_(4)were formed respectively to study the haploid induction rate and doubling rate.High-frequency inducement line Danyou 3 was used to induce haploid of backbone inbred lines and inbred progeny with different genetic bases,and the haploid seeds of each generation were doubled with colchicine concentration of 0.06%.The results showed that there were significant differences in the induction rate among inbred lines of different germplasm groups.Lancaster and Reid groups PH4CV,Mo17,PH6WC,Zheng 58 had higher induction rate.The high induction rate groups also had higher induction rate through intra-group and inter-group combination of basic materials.At the same time,the utilization of heterosis improved the induction rate of LRC,SPT and PB groups.The induction rate decreased significantly with the increase of inbred generation,and the best effect was doubled in F_(2)generation.

关 键 词：玉米 单倍体 诱导率 加倍率 种质类群 世代 

分 类 号：S513.035.2[农业科学—作物学]