甜玉米中F_(2:3)群体可溶性糖和子粒嫩度性状的数量性状位点(QTL)定位  

QTL Mapping for Soluble Sugar and Kernel Tenderness Traits Using Sweet Corn F_(2:3)Populations

作　　者：党冬冬 秦涛 于典司[1] 顾炜 阮燕晔[2] 郑洪建[1] 王慧[1] 关媛[1] DANG Dong-dong;QIN Tao;YU Dian-si;GU Wei;RUAN Yan-ye;ZHENG Hong-jian;WANG Hui;GUAN Yuan(Crop Breeding&Cultivation Research Institution/CIMMYT-China Specialty Maize Research Center,Shanghai Engineering Research Center of Specialty Maize,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai Academy of Agricultural Sciences,Shanghai 201403;College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]上海市农业科学院作物育种栽培研究所/CIMMYT-中国特用玉米研究中心/上海特用玉米工程技术研究中心/上海市农业遗传育种重点实验室,上海201403 [2]沈阳农业大学生物科学技术学院,沈阳110866

出　　处：《玉米科学》2024年第2期30-38,共9页Journal of Maize Sciences

基　　金：上海市科技兴农项目(沪农科创字[2019]第1-1号);上海市科技支撑项目(20392000400);国家玉米产业技术体系项目(CARS-02-86);上海市现代农业产业技术体系项目(沪农科产字[2017]第10号);上海市农业科学院农业科技创新支撑领域研究专项。

摘　　要：利用甜玉米自交系SHL03和SHL01为亲本构建的F_(2:3)群体,用离子色谱法(IC)测定葡萄糖(GLU)、果糖(FRU)、麦芽糖(MAL)和蔗糖(SUC)的含量,用SMS测定果肉硬度(FH)、种皮硬度(PH)、种皮脆性(PB)和果肉紧实度(FM)。结果表明,总共定位到与可溶性糖相关的QTL 34个,子粒柔嫩度相关的QTL 28个,位于1、2、3、4、5、6、7、8、10号染色体上,分别解释表型变异的1%~15.8%。主效QTL qFH3-1、qFH3-2、qFH5-2、qFH5-3、qGLU2-1、qGLU2-2的表型贡献率分别为12.2%、11.4%、12.1%、10.5%、15.8%和12.8%。在主效QTL的置信区间共找到44个注释基因,这些候选基因和相关的QTL为利用分子标记辅助选择具有更好可溶性糖含量的玉米材料和相关功能基因的克隆提供资源。In this study,the F_(2:3)population was constructed using the sweet corn inbred lines SHL03 and SHL01 as parents.The contents of glucose(GLU),fructose(FRU),maltose(MAL)and sucrose(SUC)were determined by ion chromatography(IC),and flesh hardness(FH),pericarp hardness(PH),pericarp brittleness(PB)and firmness(FM)were determined by SMS TA.XT plus texture analyzer.A total of 34 QTL related to soluble sugar,28 QTL related to grain tenderness,located in chromosome 1,2,3,4,5,6,7,8,and 10,explaining 1%-15.8%of the phenotypic variation,respectively.The phenotypic contribution rates of the major QTLs qFH3-1,qFH3-2,qFH5-2,qFH5-3,qGLU2-1,and qGLU2-2 were 12.2%,11.4%,12.1%,10.5%,15.8%,and 12.8,respectively.Within the confidence intervals of the major QTLs,a total of 44 annotated genes were identified,and these candidate genes and related QTLs provided resources for molecular marker-assisted selection of corn materials with better soluble sugar content and clones of related functional genes.

关 键 词：甜玉米 可溶性糖 子粒柔嫩性 QTL分析 候选基因 

分 类 号：S513.035.3[农业科学—作物学]