MHSeqTyper47试剂盒在亲缘关系鉴定中的应用效能评估  

Evaluating the application efficiency of the MHSeqTyper47 kit in kinship identification

作　　者：檀莉帅 金鑫[3] 冯耀森 康克莱 马温华 李明广[4] 张驰 赵杰 叶健 王乐 Tan Lishuai;Jin Xin;Feng Yaosen;Kang Kelai;Ma Wenhua;Li Mingguang;Zhang Chi;Zhao Jie;Ye Jian;Wang Le;无(School of Forensic Medicine,Shanxi Medical University,Shanxi,Jinzhong 030600,China;Key Laboratory of Forensic Genetics of Ministry of Public Security,Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;Hainan Provincial Public Security Bureau,Hainan,Haikou 570311,China;Criminalistic Science and Technology Institute of Dalian Public Security Bureau,Liaoning,Dalian 116000,China)

机构地区：[1]山西医科大学法医学院,山西晋中030600 [2]法医遗传学公安部重点实验室,公安部鉴定中心,北京100038 [3]海南省公安厅,海南海口570311 [4]大连市公安局刑事科学技术研究所,辽宁大连116000

出　　处：《中国法医学杂志》2024年第1期75-81,共7页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(81971797);公安部技术研究计划重点项目(2017JSYJA05);公安部科技强警基础工作专项(2022JC09)。

摘　　要：目的探讨MHSeqTyper47试剂盒在亲缘关系鉴定中的应用价值。方法采用MHSeqTyper47试剂盒对113份亲缘关系个体DNA样本进行基因座复合扩增及文库构建,使用MiSeq FGx测序仪进行测序,测得的数据应用MHTyper数据分析软件进行基因分型,计算亲缘关系指数评估该试剂盒在亲缘关系鉴定中的应用效能并与GlobalFiler^(TM)试剂盒进行比较。结果MHSeqTyper47在三联体鉴定中CPI值1.43×10^(11)~6.15×10^(18),二联体鉴定中CPI值3.75×10^(5)~1.53×10^(13),全同胞鉴定中CFSI值7.73×10^(1)~2.59×10^(16),三联体、二联体和全同胞关系对均与无关个体对可以完全区分。MHSeqTyper47和GlobalFiler^(TM)试剂盒联用在二级亲缘关系中的系统效能为0.4662。结论MHSeqTyper47混合DNA鉴定试剂盒在一级亲缘关系鉴定中有较高的应用价值;与GlobalFiler^(TM)试剂盒联用在二级亲缘关系鉴定中可以解决部分案例。Objective To investigate the application value of the MHSeqTyper47 kit in kinship identification.Methods Multiplexed amplification and library preparation were performed for DNA samples from 113 related individuals by using the MHSeqTyper47 kit.The libraries were sequenced on a MiSeq FGx sequencer,and the data were analyzed using MHTyper for microhaplotype genotyping.The kinship indexes were calculated to evaluate the application efficiency of this kit in kinship identification and compared with those of the GlobalFilerTM kit.Results For the MHSeqTyper47 kit,the CPI values in trio identification were 1.43×10^(11)~6.15×10^(18).The CPI values in duo identification were 1.02×10^(5)~1.53×10^(13).The CFSI values in full sibling identification were 7.73×10^(1)~2.59×10^(16).Trios,duos and full siblings could be completely distinguished from unrelated pairs.The combined efficiency of these two kits in 2nd-degree kinship identification was 0.4662.Conclusion The application value of MHSeqTyper47 kit is relatively higher in the identification of 1st-degree kinships.If jointly used with the GlobalFiler^(TM)kit,2nd-degree kinship identification could be achieved in somecases.

关 键 词：法医遗传学 微单倍型 亲缘关系鉴定 MHSeqTyper47试剂盒 ITO法 

分 类 号：D919.2[医药卫生—法医学]