环境因子对西藏野生手掌参根际土壤细菌群落结构的影响  

Effects of environmental factors on bacterial community structure in rhizosphere soil of wild Gymnadenia conopsea in Tibet

作　　者：尹秀 张二豪 李谊 普姆 刘亚可 禄亚洲 兰小中 YIN Xiu;ZHANG Er-hao;LI Yi;PU Mu;LIU Ya-ke;LU Ya-zhou;LAN Xiao-zhong(Food Science College,Tibet Agriculture and Animal Husbandry University,Nyingchi,Tibet 860000,China)

机构地区：[1]西藏农牧学院食品科学学院,西藏林芝860000

出　　处：《西南农业学报》2024年第4期835-843,共9页Southwest China Journal of Agricultural Sciences

基　　金：西藏自治区本级本门预算项目(2019-44);第四次全国中药资源普查西藏自治区中(藏)药资源普查项目(20200501-542301);中央财政支持地方高校改革发展资金-生物技术一流专业建设项目(2022-007)。

摘　　要：[目的]探究环境因子对西藏野生手掌参根际土壤细菌群落结构的影响。[方法]选取废弃木材厂(FM)、林芝观景台(LZ)、林海观景台(LH)和雷达站(LD)4个采样点采集土壤样品,采用高通量测序技术分析不同海拔手掌参根际土壤细菌群落多样性和群落结构特征及不同环境因子对其多样性和结构的影响。[结果]所有样品共检测32门、90纲、224目、378科和693属细菌,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria)为优势门,慢生根瘤菌属(Bradyrhizobium)和金黄杆菌属(Chryseobacterium)为优势属。随着海拔的升高,手掌参根际土壤细菌群落多样性和丰富度增加,且不同采样点土壤细菌群落多样性的组间差异大于组内差异。[结论]海拔、总钾(TK)、pH和电导率(EC)显著影响根际土壤细菌群落在门水平的组成,总钾(TK)、总磷(TP)和海拔3种环境因子对土壤细菌群落组成在属水平上的影响更明显。[Objective]The present paper aimed to explore the effects of environmental factors on bacterial community structure in rhizosphere soil of wild Gymnadenia conopsea in Tibet.[Method]Therefore,four sampling sites were selected to collect soil samples,including the aban-doned timber factory(FM),Linzhi viewing station(LZ),Linhai viewing station(LH),and the radar station(LD),and the high-throughput sequencing technology was used to analyze the diversity and structure of bacterial community in rhizosphere soil of G.conopsea at different al-titudes,and the effects of different environmental factors on the diversity and structure of bacterial community were analyzed.[Result]The to-tal of 32 phyla,90 classes,224 orders,378 families,and 693 genera were detected in all samples.Proteobacteria,Actinobacteria and Ac-idobacteria were the dominant phyla,Bradyrhizobium and Chryseobacterium were the dominant genera.With the increase of altitude,the diver-sity and richness of rhizosphere soil bacterial community increased,and the difference between groups was greater than the difference within groups.[Conclusion]Altitude,TK,pH and EC significantly affected the bacterial community composition in rhizosphere soil at phylum level.Three environmental factors,namely TK,TP and altitude,had more obvious effects on bacterial community composition at genus level.

关 键 词：手掌参 环境因子 根际土壤细菌 群落多样性 群落结构 

分 类 号：S154.3[农业科学—土壤学]