节拍剂量卡培他滨抑制大鼠肝移植急性排斥反应的实验观察  

Experimental observation on the inhibitory effect of metronomic capecitabine on acute rejection in rat liver transplantation

作　　者：李姗霓[1] 陈涛 王政禄[3] 郑虹[1] Li Shanni;Chen Tao;Wang Zhenglu;Zheng Hong(Department of Transplantation Oncology,Tianjin First Central Hospital,Tianjin 300192,China;School of Medicine,Nankai University,Tianjin 300071,China;Tianjin First Central Hospital Biological Sample Library,Tianjin 300192,China.)

机构地区：[1]天津市第一中心医院移植肿瘤科,天津300192 [2]南开大学医学院,天津300071 [3]天津市第一中心医院生物样本库,天津300192

出　　处：《实用器官移植电子杂志》2024年第3期247-249,共3页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：天津市卫生健康委员会科技项目(ZC20089)。

摘　　要：目的观察节拍剂量卡培他滨(capecitabine,CAP)对大鼠肝移植急性排斥反应免疫细胞及排斥反应的影响,探讨其免疫抑制作用。方法建立大鼠肝移植急性排斥反应模型,分为对照组(CON组)、节拍剂量CAP组(CAP组),节拍剂量CAP+他克莫司(tacrolimus,Tac)组(CAP+Tac组),分别于肝移植术后第1、3、7天采集静脉血,采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法检测IL-2和IFN-γ含量,分析组间白细胞介素-2(interleukin-2,IL-2)、γ-干扰素(γ-interferon,IFN-γ)变化趋势及相关性。结果肝移植术后第1、3、7天,CAP组和CAP+Tac组血清IL-2和IFN-γ含量较对照组逐渐降低,较CON组有统计学差异(P<0.05)。结论节拍剂量CAP可降低大鼠肝移植急性排斥反应期外周血IL-2和IFN-γ的分泌,具有免疫抑制作用。Objective To observe the effects of metronomic capecitabine(CAP)on immune cells and rejection in acute liver transplant rejection in rats,and to explore its immunosuppressive effect.Methods A rat liver transplantation acute rejection model was established and randomly divided into a control group(CON group),a metronomic capecitabine group(CAP group),and a metronomic capecitabine+tacrolimus(Tac)group(CAP+Tac group).Venous blood was collected on the 1st,3rd,and 7th day after liver transplantation,and enzyme-linked immunosorbent assay(ELISA)was used to detect IL-2 and IFN-γconcentrations,the trends of changes and correlations of IL-2 and IFN-γbetween groups.Results Compare to the CON group,the concentration of serum IL-2 and IFN-γgradually decreased in the CAP and CAP+Tac groups on the 1st,3rd,and 7th day after transplantation,and there was a statistically significant difference between the these two groups and the CON group(P<0.05).Conclusion The metronomic capecitabine can reduce peripheral blood IL-2 and IFN-γduring acute rejection of liver transplantation in rats,it has potential immunosuppressive effects.

关 键 词：卡培他滨 肝移植 急性排斥反应 免疫抑制 

分 类 号：R65[医药卫生—外科学]