N-羟基琥珀酰亚胺作为(pq)_(2)Ir(acac)共反应剂电致化学发光法检测Hg^(2+)  

N-hydroxysuccinimide as coreactant of(pq)_(2)Ir(acac)for the detection of Hg^(2+)by electrochemiluminescence

作　　者：付云峰 任美娟 吴芳辉[1,2] 叶明富 殷国霞[2] 王晨 FU Yunfeng;REN Meijuan;WU Fanghui;YE Mingfu;YIN Guoxia;WANG Chen(School of Chemistry and Chemical Engineering,Anhui University of Technology,Ma'anshan 243000,China;Engineering Research Center of Biofilm Water Purification and Utilization Technology of Ministry of Education,Civil Engineering&Architecture,Anhui University of Technology,Ma'anshan 243000,China;Information Materials and Devices Applications Key Laboratory of Sichuan Provincial Universities,Chengdu University of Information Technology,Chengdu 610225,China)

机构地区：[1]安徽工业大学化学与化工学院,马鞍山243000 [2]生物膜法水质净化及利用技术教育部工程研究中心,安徽工业大学建筑工程学院,马鞍山243000 [3]信息材料及器件化应用四川省高校重点实验室,成都信息工程大学,成都610225

出　　处：《分析试验室》2024年第5期642-648,共7页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21771003);生物膜法水质净化及利用技术教育部工程研究中心开放基金(BWPU2022KF04);国家级大学生创新创业训练计划项目(202210360035)资助。

摘　　要：制备了(pq)_(2)Ir(acac)(pq=2-苯基喹啉,acac=乙酰丙酮)并对其进行表征。采用N-羟基琥珀酰亚胺(NHS)作为中性混合溶剂中(pq)_(2)Ir(acac)的共反应剂,获得强的电致化学发光(ECL)信号。向该体系中加入Hg^(2+)后,因NHS与Hg^(2+)的特异性螯合作用,阻断了NHS与(pq)_(2)Ir(acac)之间的共反应,使得其ECL响应显著降低,据此构建了灵敏检测Hg^(2+)的方法。优化条件下,(pq)_(2)Ir(acac)/NHS体系ECL强度变化的对数值与Hg^(2+)浓度的自然对数值在0.1~90μmol/L范围内呈良好的线性关系,检出限达33 nmol/L。(pq)_(2)Ir(acac)/NHS体系对Hg^(2+)具有良好的选择性和稳定性。该方法可用于测定实际水样中Hg^(2+)浓度。(pq)_(2)Ir(acac)(pq=2-phenylquinoline,acac=acetylacetone)was synthesized and characterized.A strong electrochemiluminescence(ECL)signal was obtained while N-hydroxysuccinimide(NHS)exploited as an efficient coreactant for(pq)_(2)Ir(acac)ECL in mixed neutral medium.Then a highly sensitive ECL assay in a"turn-off"mode for the detection of Hg^(2+)was developed based on the specific chelation of NHS and Hg^(2+),which inhibited the reaction between NHS and(pq)_(2)Ir(acac).Under the optimized conditions,the logarithm of the ECL intensity change of the(pq)_(2)Ir(acac)/NHS system and the natural logarithm of the Hg^(2+)concentration showed a good linear relationship in the range of 0.1-90μmol/L,and the detection limit of Hg^(2+)was 33 nmol/L.This method was applied to determine Hg^(2+)in real water samples with good recovery.

关 键 词：环金属铱配合物 N-羟基琥珀酰亚胺 电致化学发光 汞离子 检测 

分 类 号：O657.3[理学—分析化学] R123.3[理学—化学]