不同消毒液对寒地早粳稻花药离体培养结果的影响  

Effects of Different Disinfectants on the Results of in Vitro Culture of Early Japonica Rice Anther in Cold Area

作　　者：刘宇 姜新龙 姜玉伟 陆文静[3] 刘辉[3] 张献国[3] 庞欢欢 张海建 LIU Yu;JIANG Xin-long;JIANG Yu-wei;LU Wen-jing;LIU Hui;ZHANG Xian-guo;PANG Huan-huan;ZHANG Hai-jian(Heilongjiang Agricultural Vocational and Technical College,Jiamusi Heilongjiang 154007,China;Jiamusi Longgeng Seed Industry Co.,LTD.,Jiamusi Heilongjiang 154026,China;Rice Research Institute,Heilongjiang Academy of Agricultural Sciences,Jiamusi Heilongjiang 154025,China)

机构地区：[1]黑龙江农业职业技术学院,黑龙江佳木斯154007 [2]佳木斯龙粳种业有限公司,黑龙江佳木斯154026 [3]黑龙江省农业科学院水稻研究所,黑龙江佳木斯154025

出　　处：《北方水稻》2024年第3期15-17,共3页North Rice

摘　　要：花药离体培养培育的植株是纯合体,可以在早世代选出所期望的性状并稳定遗传,使育种年限降低。花药离体培养操作过程中,消毒处理很重要。试验选用不同消毒液对F_(1)代植株进行处理,结果表明:84消毒液和新洁尔灭都对F_(1)代植株起到了一定的消毒作用,花药污染率分别为6.67%和7.78%,愈伤诱导率超过50%,均正常分化出绿苗。The plants cultivated by anther in vitro culture are homozygotes,which can select the desired traits in early generation and inherit them stably,thus reducing the breeding life.Disinfection is very important in the process of anther culture in vitro.In the experiment,different disinfectants were used to treat F_(1) generation plants.The results showed that both the 84 disinfectant and the Xinjieermie had certain disinfection effect on F_(1) generation plants,the anther contamination rate was 6.67%and 7.78%respectively,the callus induction rate was more than 50%,and green seedlings were differentiated normally.

关 键 词：消毒液 花药离体培养 水稻 

分 类 号：S511.22[农业科学—作物学]