地红方通过miR-133b/DUSP1/JNK通路干预糖尿病肾病氧化应激损伤  

Study on the Intervention of Didong Decoction on Oxidative Stress Injury of Diabetic Nephropathy through miR-133b/DUSP1/JNK Pathway

作　　者：林姿 何卫东[1] 杜思哲[1] LIN Zi;HE Wei-dong;DU Si-zhe(The People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China)

机构地区：[1]福建中医药大学附属人民医院,福建福州350004

出　　处：《云南中医中药杂志》2024年第5期80-84,共5页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：福建省自然科学基金(2021J01896);福建省卫生健康青年科研课题(2021QNA054)。

摘　　要：目的研究地红方对糖尿病肾病细胞模型氧化应激损伤的影响。方法地红方含药血清制备,小鼠肾小球系膜细胞常规培养、传代。按以下分组:对照组(5.5mmol/L葡萄糖+正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、地红方低剂量组(30mmol/L葡萄糖+5%地红方含药血清)、地红方中剂量组(30mmol/L葡萄糖+10%地红方含药血清)、地红方高剂量组(30mmol/L葡萄糖+20%地红方含药血清)。采用实时荧光定量PCR检测miR-133b、DUSP1 mRNA表达。Western blotting检测DUSP1、p-JNK、Drp1、Fis1蛋白表达。Elisa检测SOD、MDA表达。结果与高糖组比较,地红方组miR-133b、MDA、p-JNK、Drp1、Fis1表达明显下降(P<0.05),而DUSP1、SOD表达显著升高(P<0.05)。结论地红方可通过miR-133b/DUSP1/JNK通路调控线粒体分裂改善糖尿病肾病氧化应激损伤。Objective:To study the effect of Dihong Decoction on oxidative stress injury of diabetic nephropathy cell model.Methods:The serum containing Dihong Decoction was prepared,and mouse mesangial cells were cultured and passed through routine.Groups were divided as follows,control group(5.5mmol/L glucose+normal serum),high glucose group(30mmol/L glucose+normal serum),low dose Dihong Decoction group(30mmol/L glucose+5% Dihong Decoction containing serum),medium dose Dihong Decoction group(30mmol/L glucose+10% Dihong Decoction containing serum),and high dose Dihong Decoction group(30mm ol/L glucose+20% Dihong Decoction containing serum).Real-time fluorescent quantitative PCR was used to detect the mRNA expression of miR-133b and DUSP1.Western blotting to detect the expression of DUSP1,p-JNK,Drp1 and Fis1 proteins.The expressions of SOD and MDA were detected by Elisa.Results:Compared with that of the high glucose group,the expressions of miR-133b,MDA,P-JNK,Drp1 and Fis1 in Dihong Decoction groups were significantly decreased(P<0.05),while the expressions of DUSP1 and SOD were significantly increased(P<0.05).Conclusion:Dihong Decoction can regulate mitochondrial division through miR-133b/DUSP1/JNK pathway to improve oxidative stress injury in diabetic nephropathy.

关 键 词：地红方 糖尿病肾病 miR-133b DUSP1 JNK 氧化应激 

分 类 号：R587.1[医药卫生—内分泌]