抗IL-20单抗7E在金黄色葡萄球菌肺炎小鼠中的抗炎和肺保护作用  

Anti-inflammatory and lung protective effects of anti-IL-20 mAb 7E in mice with staphylococcus aureus pneumonia

作　　者：杨锋 陈彬彬 冯驰 Yang Feng;Chen Binbin;Feng Chi(Department of Pharmacy,Hunan People’s Hospital,Changsha 410016,China;No.5 Departments of Respiratory and Critical Care,Hunan People’s Hospital,Changsha 410016,China;Department of Respiratory and Critical Care Medicine,The Second Hospital of Huangshi,Huangshi 435000,China)

机构地区：[1]湖南省人民医院药学部,长沙410016 [2]湖南省人民医院呼吸与危重科五科,长沙410016 [3]黄石市第二医院呼吸与危重症医学科,黄石435000

出　　处：《中国组织化学与细胞化学杂志》2024年第1期19-24,共6页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的研究抗IL-20单抗在金黄色葡萄球菌(staphylococcus aureus,SA)肺炎中的作用。方法小鼠随机分为对照组、模型组、抗IL-20单抗7E干预组(7E)和万古霉素阳性对照组(Vanc)。通过气管滴注法建立小鼠SA肺炎小鼠模型,干预组在造模后通过尾静脉注射给药。收集外周血,并对外周血中白细胞和中性粒细胞计数,ELISA法检测血清中CRP、IL-20、TNF-α、IL-6和IL-1β水平;HE染色观察肺组织病理形态变化(包括肺泡结构完整性、肺泡间隔厚度、炎症、坏死及炎性细胞浸润情况),免疫组织化学和Westernblot检测小鼠肺组织中TLR2、NF-κBp65、p-STAT3的表达。结果与对照组比较,模型组肺发生明显病理改变,而7E组和Vanc组肺组织病理形态明显改善,治疗第3天开始肺泡恢复,无明显炎性细胞浸润。治疗后第4 d,7E组和Vanc组外周血中白细胞和中性粒细胞计数、血清中IL-20、TNF-α、IL-6和IL-1β含量以及肺组织中TLR2、NF-κBp65、p-STAT3的表达均明显降低。结论抗IL-20单抗7E可能通过抗炎作用延缓SA的进展,其机制可能与抑制STAT3以及TLR2/NF-κB通路有关。Objective To study the role of anti-IL-20 monoclonal antibody in staphylococcus aureus(SA)pneumonia.Meth-ods Mice were randomly divided into control group,model group,anti-IL-20 monoclonal antibody 7E intervention group(7E),and vancomycin positive control group(Vanc).A mouse model of SA pneumonia was established by tracheal instillation.The intervention group was administered medication via tail vein injection after modeling.Peripheral blood was collected,and white blood cell and neutrophil counts were measured in peripheral blood,serum levels of CRP,IL-20,TNF-α,IL-6,and IL-1βwere detected by ELISA;HE staining observed the pathological morphological changes in lung tissue(including the integrity of alveolar structure,thickness of alveolar septa,inflammation,necrosis,and inflammatory cell infiltration),immunohistochemistry and Western blot were used to detect the expression of TLR2,NF-κB p65,and p-STAT3 in mouse lung tissue.Results Compared with the control group,the model group showed significant pathological changes in the lungs,while the 7E group and Vanc group showed significant improvement in lung tissue morphology,with alveoli beginning to recover from day 3 of treatment,with no significant inflammatory cell infiltration.On day 4 after treatment,the counts of white blood cells and neutrophils in peripheral blood,the levels of IL-20,TNF-α,IL-6,and IL-1βin serum,and the expression of TLR2,NF-κB p65,and p-STAT3 in lung tissue were significantly reduced in both the 7E and Vanc groups.Conclusion The anti-IL-20 monoclonal antibody 7E may delay the progression of SA through its anti-inflammatory effects,which may be related to the inhibition of the STAT3 and TLR2/NF-κB pathways.

关 键 词：金黄色葡萄球菌肺炎 IL-20 STAT3 TLR2 NF-ΚB 

分 类 号：R563.1[医药卫生—呼吸系统]