CO_(2)干式生物甲烷化试验研究  

Experimental study on CO_(2)dry biomethanation

作　　者：谢昕 王春辉 于荣珍 徐恒 周昊[2] 孙志明[2] 王建兵[2] XIE Xin;WANG Chunhui;YU Rongzhen;XU Heng;ZHOU Hao;SUN Zhiming;WANG Jianbing(Shandong Benyuan Environmental Technology Co.,Ltd.,Jinan 250100,China;School of Chemistry and Environmental Engineering,China University of Mining and Technology(Beijing),Beijing 100083,China)

机构地区：[1]山东本源环境科技股份有限公司,山东济南250100 [2]中国矿业大学(北京)化学与环境工程学院,北京100083

出　　处：《能源环境保护》2024年第3期109-116,共8页Energy Environmental Protection

基　　金：国家自然科学基金资助项目(51978658)。

摘　　要：随着我国逐步成为最大能源消费国与碳排放国,CO_(2)的高效资源化利用成为实现“双碳”战略的重要手段。微生物催化还原CO_(2)备受关注,可低成本同步实现绿氢储存和CO_(2)转化利用。围绕CO_(2)干式生物甲烷化方法的建立,从转化速率的关键影响因素和微生物群落结构两方面开展实验研究。研究结果表明,在无氧干式反应器中,以载碳海绵为填料,温度设置为35℃,最佳H_(2)/CO_(2)进料比为4∶1,最佳营养液浸泡周期为6 d。通过高通量测序技术对浸泡周期结束的微生物进行测序分析,发现在干式反应器中,厌氧耗氢产甲烷菌是主导,属水平上以Methanobacterium和Methanosaeta为主,细菌群落中Acetobacterium和Mesotoga丰富度占比较高。对CO_(2)干式生物甲烷化方法的转化速率的关键影响因素和微生物群落结构进行研究,为微生物产甲烷提供了理论基础和技术支撑。As China has gradually become the largest energy consumer and carbon emitter,the efficient utilization of CO_(2)has become an important means in realizing the"dual carbon"strategy.Microbial catalytic reduction of CO_(2)offers a low-cost solution for simultaneous green hydrogen storage and CO_(2)conversion and utilization.This paper focuses on the establishment of CO_(2)dry biomethanization method,and conducts experiments to identify the key factors affecting the conversion rate and microbial community structure.The results showed that maintaining a temperature of 35℃,employing an optimal H_(2)/CO_(2)feed ratio of 4∶1,and implementing a 6-day soaking period for the nutrient solution in an anaerobic dry reactor filled with carbon sponge are critical for enhancing the conversion rate.Additionally,microbes at the end of the soaking cycle were sequenced and analyzed by high-throughput sequencing technology.The dry reactor exhibited dominance by anaerobic hydrogen-consuming methanogens,particularly at the genus level with Metanobacterium and Metaosaeta being prevalent.Moreover,the bacterial community displayed relatively high levels of Aconobacterium and Mesotoga.This study aims to investigate the key factors affecting the conversion rate of CO_(2)dry anaerobic biomethanation and to analyze the structure of the microbial community,in order to provide a theoretical foundation and technical support for the process of microbial methane production.

关 键 词：二氧化碳 氢气 关键影响因素 微生物群落 干式生物甲烷化 

分 类 号：X51[环境科学与工程—环境工程] X701