海南省福寿螺系统发育分析及种群遗传学研究  

Phylogenetic analysis and population genetics of the Pomacea snail(Gastropoda:Ampullariidae)in Hainan province,China

作　　者：吕鑫 Jitrawadee Intirach 黄萍 张帆[1] 高素真[1] 焦惠生 舒畅 周云飞 吕志跃 LüXin;Jitrawadee Intirach;HUANG Ping;ZHANG Fan;GAO Suzhen;JIAO Huisheng;SHU Chang;ZHOU Yunfei;LüZhiyue(School of Public Health,Hainan Medical University,Haikou,Hainan 571199,China;Department of Scientific Research,Hainan General Hospital,Hainan Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570100,China;School of Tropical Medicine,Hainan Medical University,Haikou,Hainan 571199,China;Department of Pathogen Biology,Medical College of Soochow University,Suzhou,Jiangsu 215031,China;School of Basic Medical Sciences and Life Sciences,Hainan Medical University,Haikou,Hainan 571199,China;Key Laboratory of Tropical Disease Control of Ministry of Education,Sun Yat-sen University,Guangzhou,Guangdong 510080,China)

机构地区：[1]海南医学院公共卫生学院,海南海口571199 [2]海南省人民医院,海南医学院附属海南医院科研部,海南海口570100 [3]海南医学院热带医学与检验医学院,海南海口571199 [4]苏州大学医学院病原生物学教研室,江苏苏州215031 [5]海南医学院基础医学与生命科学学院,海南海口571199 [6]中山大学热带病防治教育部重点实验室,广东广州510080

出　　处：《热带医学杂志》2024年第4期451-459,F0002,共10页Journal of Tropical Medicine

基　　金：国家自然科学基金(82072303,81572023,81371836);国家重点研发计划(2021YFC2300800,2021YFC23008001,2021YFC23008002,2021YFC23008003);海南省重大科技计划项目(ZDKJ202003,ZDKJ2021035);海南省重点研发计划项目(ZDYF2020120);国家寄生虫资源库(NPRC-2019-194-30);广东省科技计划项目(2019B030316025);广东省自然科学基金(2019A1515011541)。

摘　　要：目的对海南省福寿螺进行分子鉴定,完成遗传多样性和亲缘关系研究,为识别和防控福寿螺的持续扩散提供依据。方法基于线粒体细胞色素c氧化酶I(COI)和16S核糖体脱氧核糖核酸(16S rDNA)基因,对海南省18个县市61个采样点采集的福寿螺样本,进行系统发育分析及种群遗传学研究。采集1050只福寿螺,随机对其中632只和577只进行COI和16S rDNA基因测序。从GenBank数据库下载序列构建数据库,COI和16S rDNA数据库分别有1465和641条序列。使用MEGA X和DnaSP软件进行系统发育和遗传多样性分析。结果小管福寿螺为海南省优势种(79.9%)。小管福寿螺和隐秘福寿螺在海南17个市县同时存在。基于COI基因,小管福寿螺分为A支(与来自日本和中国福寿螺亲缘关系密切)、B支(与来自日本、菲律宾、马来西亚和中国福寿螺亲缘关系接近)和C支(与来自马来西亚、日本、阿根廷、巴布亚新几内亚、缅甸、智利和中国福寿螺亲缘关系近)。基于16S rDNA基因,小管福寿螺分为A支和B支系(与来自马来西亚、阿根廷、菲律宾、美国和中国福寿螺亲缘关系较近)。单倍型网络分析表明,基于小管福寿螺的COI和16S rDNA基因,分别有4个和3个单倍型。隐秘福寿螺只表现出单一的COI单倍型。保亭黎族苗族自治县的福寿螺样本单倍型和核苷酸多样性最高(Hd=0.679,π=0.053),而琼海市和屯昌县之间的种群分化最大(F_(ST)=0.63)。小管福寿螺和隐秘福寿螺的遗传多样性较低。结论海南省小管福寿螺的COI和16S rDNA序列具有多态性。福寿螺可能被多次引入海南,其中隐秘福寿螺在海南省首次得到确认,尚未记录到斑点福寿螺的分布。海南省小管福寿螺和隐秘福寿螺的遗传多样性较低。Objective Molecular identification of the Pomacea snails in Hainan province was carried out to complete the study of genetic diversity and genetic relationships,which could provide a basis for identifying and preventing the continued spread of the Pomacea snails.Methods Based on mitochondrial cytochrome c oxidase I(COI)and 16S ribosomal deoxyribonucleic acid(16S rDNA)genes,phylogenetic analysis and population genetics studies were conducted on Pomacea snail samples collected from 61 sampling points in 18 counties and cities in Hainan province.From the collected 1050 Pomacea snails,632 and 577 of them were sequenced for COI and 16S rDNA genes.The relevant sequences were downloaded from the GenBank database to construct the database.The COI and 16S rDNA databases had 1465 and 641sequences respectively.Phylogenetic and genetic diversity analyzes were performed using MEGA X and DnaSP software.Results Pomacea canaliculata was found to be a dominant species(79.9%).The sympatric occurrence of P.canaliculata and P.occulta in 17 cities and counties was recorded.P.can.aliculata isolates were categorized(based on COI)into clade A(closely related to strains from Japan and China),B(displaying proximity to strains from Japan,the Philippines,Malaysia and China),and C(closely related to strains from Malaysia,Japan,Argentina,Papua New Guinea,Myanmar,Chile and China).P.canaliculata was classified(based on 16S rDNA)into clade A and B(closely related to strains from Malaysia,Argentina,the Philippines,the USA and China).The haplotype network analysis revealed that P.canaliculata could be grouped into four and three haplotypes,corresponding to COI and 16S rDNA gene assemblages,respectively.Conversely,P.occulta exhibited only a single COI haplotype.Moreover,Pomacea specimens from Baoting Li and Miao Autonomous County had the highest haplotype and nucleotide diversities(Hd=0.679,π=0.053),whereas differentiation of the largest pairwise population existed between Qionghai city and Tunchang county(F_(ST)=0.63).The two Pomacea species had

关 键 词：遗传多样性 系统发育分析 单倍型 福寿螺 海南省 

分 类 号：Q958[生物学—动物学]