吉林省水稻恶苗病菌对咯菌腈的敏感性及抗药性风险评估  

Sensitiveness and Resistance Risk Assessment of Rice Fusarium fujikuroi to Fludioxonil in Jilin Province

作　　者：刘禹含 邹佳营 何博 刘丽萍 高洁 LIU Yuhan;ZOU Jiaying;HE Bo;LIU Liping;GAO Jie(College of Plant Protection,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学植物保护学院,长春130118

出　　处：《吉林农业大学学报》2024年第2期211-218,共8页Journal of Jilin Agricultural University

基　　金：国家重点研发计划项目(2018YFD0300200);家级大学生创新创业训练计划项目(201810193042)。

摘　　要：为了解吉林省水稻恶苗病菌(Fusarium fujikuroi)对咯菌腈的敏感性,并评估其抗性风险。采用菌丝生长速率法,分别测定了2016—2018年,自吉林省16个地区分离获得的水稻恶苗病菌F.fujikuroi 508株,从中选取F.fujikuroi 136株测定其对咯菌腈的敏感性。通过药剂驯化方法获得F.fujikuroi抗咯菌腈突变体。结果表明:F.fujikuroi对咯菌腈的EC_(50)值分布范围为2.810×10^(-9)~0.7409 mg/L,对咯菌腈极其敏感,未发现抗药性群体,所测菌株EC_(50)平均值为(0.0249±0.0936)mg/L,表明吉林省水稻恶苗病菌对咯菌腈没有产生抗药性。药剂驯化获得2株抗药突变体,抗性倍数分别为3596.48和9539.73,表现为高抗。通过生物学测定,抗性突变体的适合度极高,易在田间成为优势菌。交互抗性试验结果表明,咯菌腈与福美双、甲基硫菌灵、精甲霜灵和噁霉灵存在正交互抗性,与戊唑醇和咪鲜胺无交互抗性。水稻恶苗病菌在药剂选择压力下可形成抗咯菌腈突变体,存在抗药性风险。研究结果为吉林省水稻恶苗病的防治及抗药性治理提供科学依据。The study aims to understand the sensitiveness of Fusarium fujikuroi to fludioxonil and assess the risk of resistance.From 2016 to 2018,508 strains of F.fujikuroi were isolated from 16 regions of Jilin province,and sensitiveness of 136 strains of F.fujikuroi to fludioxonil was determined by mycelial growth rate method.Resistant mutants of rice F.fujikuroi to fludioxonil were obtained by drug domestication.The results showed that EC_(50)values of 136 selected strains to fludioxonil ranged from 2.810×10^(-9)to 0.7409 mg/L,which were extremely sensitive to fludioxonil.No resistant population was found,and the mean EC_(50)value was(0.0249±0.0936)mg/L,indicating that rice F.fujikuroi in Jilin province did not develop resistance to fludioxonil.Compared with their parent strains,resistance levels of the two resistant mutants(LH10-2-11 and DH34-5-11)were 3596.48 and 9539.73fold,respectively,showing high resistance.Through biological testing,adaptability of resistant mutants was proved to be extremely high,making them easy to become dominant fungi in the field.The results of cross resistance test showed that there was positive cross resistance between fludioxonil and thiram,thiophanate-methyl,metalaxyl-M and hymexazd,but no cross resistance with prochloraz and tebuconazole.The results suggested that the resistant mutants of F.fujikuroi from rice can be developed under the selection of fludioxonil,which poses a risk of resistance to fludioxonil.The results lay foundation for the control of rice bakanae caused by F.fujikuroi and management of fungicide resistance of F.fujikuroi to fludioxonil.

关 键 词：水稻恶苗病菌 咯菌腈 敏感性 抗药突变体 生物学性状 抗药性治理 吉林省 

分 类 号：S435.111.44[农业科学—农业昆虫与害虫防治]