青风藤总生物碱提取工艺及抗痛风作用  

Extraction Process of Total Alkaloids from Sinomenii caulis and Its Anti Gout Effect

作　　者：曹沛莹 陈维佳 宗颖[1] 时坤[1] 李建明[1] 何忠梅[1] 杜锐[1] CAO Peiying;CHEN Weijia;ZONG Ying;SHI Kun;LI Jianming;HE Zhongmei;DU Rui(College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《吉林农业大学学报》2024年第2期280-289,共10页Journal of Jilin Agricultural University

基　　金：吉林省科技引导计划国际科技合作项目(20180414072GH)。

摘　　要：以比色法测得的青藤碱含量为评价指标,采用乙醇加热回流提取法,考察乙醇浓度、液料比、提取时间和提取次数对青风藤生物碱得率的影响,用星点设计-响应面法分析从青风藤中提取青风藤总生物碱的最佳提取工艺参数;采用氧嗪酸钾腹腔注射联合关节腔注射尿酸钠造成大鼠痛风模型,用ELISA法测定青风藤总生物碱对痛风模型大鼠血清尿酸(UA)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、黄嘌呤氧化酶(XOD)、尿素氮(BUN)、肌酐(Cr)、胱抑素c(Cys-c)、谷丙转氨酶(ALT)含量以及关节滑膜组织中UA,IL-1β,IL-6,TNF-α含量的影响;采用H&E染色法观察青风藤总生物碱对痛风模型大鼠的肾脏保护作用。星点设计-响应面法分析得到青风藤总生物碱最佳提取工艺条件:10倍量75%乙醇,加热回流提取3次,每次1.8 h;青风藤总生物碱可显著改善痛风大鼠关节炎导致的足肿胀,降低模型大鼠血清中UA,IL-1β,IL-6,TNF-α,XOD,BUN,Cr,Cys-c和ALT水平,显著降低大鼠关节滑膜组织中UA,IL-1β,IL-6,TNF-α含量。HE染色结果表明,青风藤总生物碱可减轻痛风大鼠肾损伤,减少炎性浸润。上述研究结果表明,星点设计-响应面法优化青风藤总生物碱乙醇提取工艺条件,方法简单可行,建立的数学模型预测性良好,青风藤总生物碱具有显著的抗痛风作用。Using sinomenine content measured by ultraviolet spectrophotometry as evaluation index,ethanol heating reflux extraction method was adopted to investigate the effects of ethanol concentration,liquid-to-material ratio,extraction time and extraction times on the yield of Sinomenii caulis alkaloids.The optimal extraction process parameters for extracting total alkaloids from Sinomenii caulis were analyzed using star point design-response surface method.Ethanol heating reflux and intraperitoneal injection of potassium oxyzinate combined with intra-articular injection of sodium urate were used to create a rat model of gout.The effects of total alkaloids of Sinomenii caulis on the levels of UA,IL-1β,IL-6,TNF-α,XOD,BUN,Cr,Cys-c and ALT in serum and the levels of UA,IL-1β,IL-6 and TNF-αin synovial tissue of joint were detected by ELISA.HE staining was used to observe the protective effect of alkaloids from Sinomenii caulis on kidneys in rats with gout.The optimum extraction conditions of alkaloids from Sinomenii caulis obtained by star point design-response surface method were as follows:10 times the amount of 75%ethanol,reflux extraction 3 times,1.8 h each time;The total alkaloids of Sinomenii caulis can significantly improve the paw swelling caused by gout,reduce the levels of UA,IL-1β,IL-6,TNF-α,XOD,BUN,Cr,Cys-C and ALT in the serum of the model rats,and significantly reduce the contents of UA,IL-1β,IL-6 and TNF-αin the synovium of the rats.The results of HE staining showed that the total alkaloids can reduce kidney damage and inflammatory infiltration in rats with gout.The above research results indicate that star point design-response surface method is a simple and feasible method for optimizing the ethanol extraction process of total alkaloids from Sinomenii caulis.The established mathematical model has good predictive ability,and the total alkaloids from Sinomenii caulis have a significant anti gout effect.

关 键 词：青风藤 生物碱 痛风 星点设计-响应面法 

分 类 号：S567[农业科学—中草药栽培]