人冠状病毒HCoV-229E在无生命物体表面存活时间比较研究  

Comparative study on the survival time of human coronavirus HCoV-229E on the surface of inanimate objects

作　　者：刘冷[1] 邓惠[1] 陈惠珍[1] 郑小凌[1] 王冰姝[1] 王雅静[1] 黄旭和[1] 张磊 纪迅敏 钟昱文[1] LIU Leng;DENG Hui;CHEN Hui-zhen;ZHENG Xiao-ling;WANG Bing-shu;WANG Ya-jing;HUANGXu-he;ZHANG Lei;JI Xun-min;ZHONG Yu-wen(Guangdong Provincial Center for Disease Control and Prevention,Guangzhou Guangdong 511430;Guangdong Provincial Institute of Biological Products and Materia Medica,China)

机构地区：[1]广东省疾病预防控制中心,广东广州511430 [2]广东省生物制品与药物研究所

出　　处：《中国消毒学杂志》2024年第4期253-255,共3页Chinese Journal of Disinfection

基　　金：广东省医学科研基金(202342793455200)。

摘　　要：目的比较人冠状病毒HCoV-229E在不同的无生命载体上的存活时间,为做好冠状病毒消毒提供依据。方法试验环境温度为(25±1)℃,相对湿度为55%±10%,采用细胞接种法检测病毒存活,病毒悬液滴度达到国家标准要求,进行不同物体表面上HCoV-229E的存活试验。结果纸片、布片载体上病毒滴度下降较快,滴染纸片、布片72 h后,病毒滴度接近0;塑料片载体上病毒滴度下降较慢,放置5 d病毒滴度为0.75 logTCID_(50)/0.1 mL;不锈钢载体、玻璃片载体上病毒滴度下降较慢,放置4 d后,病毒滴度分别为1.0和0.75 logTCID_(50)/0.1 mL。5 d以后所有载体上均未检测到活病毒。结论HCoV-229E在不同无生命物体表面上存活时间不同,在实际消毒工作中应有针对性地开展物体表面和环境消毒,提高消毒工作效能。Objective To compare the survival time of human coronavirus HCoV-229E on different vectors of inanimate object surfaces,and to provide evidence for coronavirus disinfection.Methods Under the conditions of test ambient temperature of(25±1)℃and relative humidity of 55%±10%,the cell inoculation method was used to detect virus survival and the titer of virus suspension reached the national standard,and the survival test of HCoV-229E on different object surfaces was conducted.Results The virus titer on the vectors of paper and cloth decreased rapidly,and was close to 0 after 72 h of instilled contamination of paper and cloth.The virus titer decreased slowly on the plastic vector,and was 0.75 logTCID_(50)/0.1 mL after 5 d.The virus titer on the vectors of stainless steel and glass sheet decreased slowly,and was 1.0 logTCID_(50)/0.1 mL and 0.75 logTCID_(50)/0.1 mL respectively after 4 days,and no live virus was detected on all vectors of inanimate object surfaces after 5 d.Conclusion The survival times of HCoV-229E on different inanimate object surfaces are different,and targeted disinfection of object surfaces and environment should be carried out in actual disinfection work to improve disinfection efficiency.

关 键 词：人冠状病毒 载体 物体表面 存活时间 

分 类 号：R117[医药卫生—公共卫生与预防医学]