荧光标记法和微生物法在重症监护室环境清洁消毒监测中的应用  

Application of fluorescent labeling method and microbiological method in the effect evaluation of environmental cleaning and disinfection in ICU

作　　者：李蓓 焦阳 茅一萍 LI Bei;JIAO Yang;MAO Yi-ping(The Affiliated Hospital of Xuzhou Medical University,Xuzhou Jiangsu 221000,China)

机构地区：[1]徐州医科大学附属医院,江苏徐州2210001

出　　处：《中国消毒学杂志》2024年第4期264-267,共4页Chinese Journal of Disinfection

基　　金：徐州医科大学附属医院发展基金自主项目(XYFC2021002)。

摘　　要：目的探索荧光标记法和微生物法在评价重症监护室(ICU)多重耐药菌感染及定植患者周围环境清洁消毒质量中的应用效果。方法针对某医院12个ICU所有耐碳青霉烯鲍曼不动杆菌感染/定植患者的周围环境,采用荧光标记法和微生物法比较环境清洁干预措施实施前后各ICU的荧光标记清除率、微生物标本合格率和鲍曼不动杆菌检出率。结果干预后荧光标记清除率由35.85%上升至66.94%,微生物标本合格率由80.57%上升至93.13%,鲍曼不动杆菌检出率由27.43%下降到8.75%,差异均有统计学意义(P<0.05)。结论荧光标记法和微生物法可用于多重耐药菌感染/定植患者的物体表面清洁消毒效果评价。Objective To explore the application effect of fluorescent labeling method and microbiological method in evaluating the quality of cleaning and disinfection of the surrounding environment of patients with multidrug-resistant organism infection/colonization in intensive care units(ICU).Methods Focusing on the surrounding environment of all patients with carbapenem-resistant Acinetobacter baumannii infection/colonization in 12 ICUs in the hospital,fluorescent labeling methods and microbiological methods were used to compare the fluorescent labeling clearance rate,the qualified rate of microbial specimens and the detection rate of Acinetobacter baumannii of each ICU before and after the implementation of environmental cleaning intervention measures.Results After the intervention,the fluorescence labeling clearance rate increased from 35.85%to 66.94%,and the qualified rate of microbial specimens increased from 80.57%to 93.13%.The detection rate of Acinetobacter baumannii decreased from 27.43%to 8.75%,and the differences were all statistically significant(P<0.05).Conclusion Fluorescent labeling method and microbiological method can be used to evaluate the effect of object surface cleaning and disinfection in patients with multidrug-resistant organism infection/colonization.

关 键 词：荧光标记法 微生物法 重症监护室 清洁消毒 效果评价 

分 类 号：R187[医药卫生—流行病学]