大黄糖络丸对2型糖尿病大鼠肝损伤的影响及机制  

Effects and mechanism of rhubarb Tangluo pill on liver injury in type 2 diabetic rats

作　　者：安冬 梁永林 高艳奎 闫丰喆 白敏 赵思晨 刘中唐 聂宵 AN Dong;LIANG Yong-lin;GAO Yan-kui;YAN Feng-zhe;BAI Min;ZHAO Si-chen;LIU Zhong-tang;NIE Xiao(School of Basic Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学基础医学院,甘肃兰州730000

出　　处：《中国临床药理学杂志》2024年第9期1282-1286,共5页The Chinese Journal of Clinical Pharmacology

基　　金：甘肃省优秀博士生基金资助项目(23JRRA1218);甘肃省高等学校产业支撑计划基金资助项目(2021CYZC-03);甘肃中医药大学研究生创新基金资助项目(2022CX05)。

摘　　要：目的探讨大黄糖络丸对2型糖尿病大鼠肝损伤的影响及机制。方法将ZDF(fa/fa)大鼠给予高脂饲料诱导2型糖尿病模型,造模成功后随机分为模型组、阳性药对照组(0.18 g·kg^(-1)二甲双胍)和低、中、高剂量实验组(0.54、1.08和2.16 g·kg^(-1)大黄糖络丸),每组8只。选取8只ZDF(fa/+)大鼠作为对照组。对照组及模型组灌服等体积纯净水,每日1次,持续12周。给药结束后进行口服葡萄糖耐量试验(OGTT);检测大鼠空腹血糖水平、体质量及肝质量并计算肝指数;检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、三酰甘油(TG)、总胆固醇(TC)含量;用苏木精-伊红(HE)染色法和马松(Masson)染色法观察肝组织形态学变化。用蛋白质印迹检测肝组织磷酸化胰岛素受体底物1(p-IRS1)、磷酸化蛋白激酶B(p-Akt)及葡萄糖转运蛋白4(GLUT4)的蛋白表达。结果对照组、模型组、阳性药对照组和高剂量实验组大鼠的空腹血糖分别为(4.71±0.45)、(29.90±2.97)、(15.28±4.52)和(13.84±1.55)mmol·L^(-1),肝指数分别为2.31±0.46、4.03±0.18、3.37±0.23和3.38±0.24,p-IRS1蛋白相对表达水平分别为1.00±0.36、4.00±0.11、1.62±0.27和1.90±0.17,p-Akt蛋白相对表达水平分别为1.00±0.25、0.21±0.04、0.73±0.15和0.54±0.04,GLUT4蛋白相对表达水平分别为1.00±0.11、0.40±0.08、0.86±0.04和0.70±0.06。高剂量实验组上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.01,P<0.05)。结论大黄糖络丸可有效改善2型糖尿病糖脂代谢和肝损伤,其作用机制可能与IRS1/Akt信号通路的激活有关。Objective To investigate the effect and mechanism of rhubarb Tangluo pill on liver injury in type 2 diabetic rats.Methods ZDF(fa/fa)rats were given high-fat diet to induce type 2 diabetes model,and were randomly divided into model group,positive control group(0.18 g·kg^(-1)metformin)and experimental-L,-M,-H groups(0.54,1.08 and 2.16 g·kg^(-1)rhubarb Tangluo pill),with 8 rats in each group.Eight ZDF(fa/+)rats were selected as control group.The control group and model group were given equal volume of pure water once a day for 12 weeks.An oral glucose tolerance test(OGTT)was performed after administration.Fasting blood glucose level,body mass and liver mass of rats were measured and liver index was calculated.The contents of glutamic-pyruvic transaminase(GPT),glutamic oxalacetic transaminase(GOT),triglyceride(TG)and total cholesterol(TC)in serum were detected.The histomorphologic changes of liver were observed by hematoxylin-eosin(HE)staining and Masson staining.The protein expression of phosphorylated insulin receptor substrate 1(p-IRS1),phosphorylated protein kinase B(p-Akt)and glucose transporter 4(GLUT4)were detected by Western blotting.Results After administration,the fasting blood glucose levels of control group,model group,positive control group and experimental-H group were(4.71±0.45),(29.9±2.97),(15.28±4.52)and(13.84±1.55)mmol·L^(-1),respectively;the liver index were 2.31±0.46,4.03±0.18,3.37±0.23 and3.38±0.24;the relative expression level of p-IRS1 protein were 1.00±0.36,4.00±0.11,1.62±0.27 and1.90±0.17,respectively;the relative expression levels of p-Akt protein were 1.00±0.25,0.21±0.04,0.73±0.15 and 0.54±0.04,respectively;GLUT4 protein relative expression levels were 1.00±0.11,0.40±0.08,0.86±0.04 and 0.70±0.06,respectively.Compared with the model group,the above indexes in the experimental-H group were statistically significant(P<0.01,P<0.05).Conclusion Rhubarb Tanglu pill can effectively improve glycolipid metabolism and liver injury in type 2 diabetes mellitus,and its mechanism ma

关 键 词：大黄糖络丸 2型糖尿病 肝损伤 胰岛素抵抗 胰岛素受体底物1/蛋白激酶B信号通路 

分 类 号：R28[医药卫生—中药学]