肉桂油对大鼠前列腺增生的治疗作用及其机制研究  

Effect and mechanism of cinnamon oil on rat prostatic hyperplasia

作　　者：何微 宋蝶 王红萍 马松涛[1] HE Wei;SONG Die;WANG Hong-ping;MA Song-tao(Department of Pharmacy,Chengdu Medical College,Chengdu 610500,Sichuan Province,China)

机构地区：[1]成都医学院药学院,四川成都610500

出　　处：《中国临床药理学杂志》2024年第9期1292-1295,共4页The Chinese Journal of Clinical Pharmacology

基　　金：成都医学院丽研工坊医美研究中心基金资助项目(22YM005);四川省科技厅基金资助项目(2015GZX0077)。

摘　　要：目的研究肉桂油对实验性大鼠前列腺增生的治疗作用及其作用机制。方法将SD大鼠随机分成正常组、模型组、对照组和肉桂油高、中、低剂量组,每组各10只。大鼠皮下注射5 mg·kg^(-1)·d^(-1)丙酸睾酮,连续30 d,诱导大鼠前列腺增生模型。正常组和模型组灌胃给予1 mL·kg^(-1)的0.9%NaCl,对照组灌胃给予1 mg·kg^(-1)非那雄胺,肉桂油高、中、低剂量组分别灌胃给予1、0.5和0.25 mg·kg^(-1)肉桂油,每天1次,连续30 d。比较大鼠前列腺湿质量和指数等指标,研究肉桂油对5α-还原酶和还原型烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶的抑制作用。结果正常组、模型组、对照组和肉桂油高、中、低剂量组大鼠前列腺湿质量分别为(0.51±0.09)、(1.02±0.17)、(0.65±0.15)、(0.64±0.17)、(0.79±0.09)和(0.81±0.15)mg,前列腺指数分别为(2.25±0.33)、(4.70±0.48)、(3.03±0.82)、(3.02±0.60)、(3.53±0.46)和(3.68±0.66)mg·kg^(-1),NADPH氧化酶荧光强度分别为(1.26±0.18)、(5.21±0.80)、(2.21±0.56)、(2.51±0.56)、(4.13±0.64)和(4.90±0.88)RLU,对照组和肉桂油高、中剂量组与模型组相比,在统计学上差异有统计学意义(P<0.01)。同时研究还发现,阳性对照组(20μmol·L^(-1)非那雄胺)、肉桂油组(10、20μmol·L^(-1))对5α-还原酶的平均抑制率分别为49.36%、45.62%和63.45%,肉桂油组(20μmol·L^(-1))对5α-还原酶的平均抑制率高于阳性对照组,在统计学上差异有统计学意义(P<0.05)。结论肉桂油可能通过抑制5α-还原酶和抑制NADPH氧化酶抑制前列腺增生。Objective To research the effect and the mechanism of cinnamon oil on experimental rat prostatic hyperplasia.Methods SD rats were randomly divided into normal group,model group,control group and cinnamon oil-H,-M,-L groups,with 10 rats in each group.The prostatic hyperplasia model was induced by injected subcutaneously with 5 mg·kg^(-1)·d^(-1)testosterone propionate for 30 d.Normal group and model group were given 1 mL·kg^(-1)0.9%NaCl;control group were given 1 mg·kg^(-1)finasteride;cinnamon oil-H,-M,-L groups were given 1,0.5,0.25 mg·kg^(-1)cinnamon oil by intragastric administration,respectively,once a day for 30 d.The wet weight and index of prostate gland in rats were compared to explore the therapeutic effect of cinnamon oil,and the inhibitory effect of cinnamon oil on 5α-reductase and NADPH oxidase was studied to explore its mechanism.Results The prostate wet weight in normal group,model group,control group and cinnamon oil-H,-M,-L groups were(0.51±0.09),(1.02±0.17),(0.65±0.15),(0.64±0.17),(0.79±0.09)and(0.81±0.15)mg;the prostate index were(2.25±0.33),(4.70±0.48),(3.03±0.82),(3.02±0.60),(3.53±0.46)and(3.68±0.66)mg·kg^(-1);the fluorescence intensity of NADPH oxidase were(1.26±0.18),(5.21±0.80),(2.21±0.56),(2.51±0.56),(4.13±0.64)and(4.90±0.88)RLU,respectively.Compared with model group,the fluorescence intensity of NADPH oxidase in normal group,control group and cinnamon oil-H,-M groups all had significantly differences(allP<0.01).The average inhibition rates of 5α-reductase in positive control group(20μmol·L^(-1)),cinnamon oil group(10,20μmol·L^(-1))were 49.36%,45.62%and 63.45%,respectively.The average inhibition rate of 5α-reductase in cinnamon oil group(20μmol·L^(-1))was higher than that in positive control group(20μmol·L^(-1)),the difference was statistically significant(P<0.05).Conclusion Cinnamon oil may inhibit prostate hyperplasia by inhibiting 5α-reductase and NADPH oxidase.

关 键 词：肉桂油 前列腺增生 5Α-还原酶 还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶 

分 类 号：R28[医药卫生—中药学]