枳术颗粒对功能性消化不良大鼠胃动力的改善作用  

Effect of Zhizhu Granules on improving gastric motility in rats with functional dyspepsia

作　　者：高仁浩 陈欢 赵开军 王海丽 成俊 黄芳 GAO Renhao;CHEN Huan;ZHAO Kaijun;WANG Haili;CHENG Jun;HUANG Fang(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China;Jiangsu Classical Prescriptions of Traditional Chinese Medicine Engineering Technology Research Center,Nanjing Zhongshan Pharmaceutical Co.,Ltd.,Nanjing 210046,China;Nanjing Clinical Experience Prescription Modernization Engineering Research Center,Jiangsu Hongdian Research Institute of Traditional Chinese Medicine Industry Co.,Ltd.,Nanjing 210023,China)

机构地区：[1]中国药科大学中药学院,江苏南京211198 [2]南京中山制药有限公司,江苏省中药经典名方工程技术研究中心,江苏南京210046 [3]江苏弘典中药产业研究院有限公司,南京市临床经验方现代化工程研究中心,江苏南京210023

出　　处：《中草药》2024年第8期2620-2629,共10页Chinese Traditional and Herbal Drugs

基　　金：企业知识产权战略推进计划项目(ZT20210180-33)。

摘　　要：目的研究枳术颗粒对功能性消化不良(functional dyspepsia,FD)大鼠的药效及作用机制。方法复合因素造模法诱导大鼠FD模型,造模成功后给予枳术颗粒治疗2周,观察枳术颗粒对FD大鼠胃残留率、小肠推进率、血清中胃泌素(gastrin,GAS)、胃饥饿素(growth hormone releasing peptide,Ghrelin)、降钙素基因相关肽(calcitonin gene related peptide,CGRP)及生长抑素(somatostatin,SS)含量的影响;苏木素-伊红(hematoxylin-eosin,HE)染色法观察胃窦组织、十二指肠组织病理变化;利用qRT-PCR检测下丘脑及胃组织中GAS、Ghrelin、CGRP、SS mRNA表达和胃及十二指肠组织中肾上腺素能受体β1(β1-adrenergic receptor,ADRB1)、生长抑素受体(somatostatin receptor,SSTR)、胃饥饿素-O-乙酰转移酶(ghrelinO-acyltransferase,GOAT)、胃饥饿素受体(growth hormone secreting hormone receptor,GHSR)、酪氨酸激酶受体(c-kit protooncogene protein,C-kit)、干细胞因子(stem cell factor,SCF)、钙激活氯离子通道蛋白Anoctamin-1(ANO1)、肌球蛋白轻链激酶(myosin light chain kinase,MLCK)m RNA表达;利用Western blotting检测胃及十二指肠组织中C-kit、SCF蛋白表达。结果枳术颗粒可以显著降低FD大鼠胃残留率(P<0.05),提高小肠推进率(P<0.01);升高血清中Ghrelin、GAS含量(P<0.01),降低CGRP、SS含量(P<0.05、0.01);升高下丘脑及胃窦组织中Ghrelin、GAS mRNA表达水平(P<0.05、0.01),降低下丘脑及胃窦组织中CGRP、SS mRNA表达水平(P<0.01);显著升高胃及十二指肠组织SCF、C-kit蛋白表达水平(P<0.05、0.01),升高胃及十二指肠组织ADRB1、GOAT、GHSR、SCF、C-kit、ANO1、MLCK mRNA表达水平(P<0.05、0.01),降低SSTR m RNA表达水平(P<0.05、0.01)。结论枳术颗粒可以改善FD大鼠的胃动力异常,其作用机制可能与调节脑肠肽水平、激活SCF/C-kit通路有关。Objective To study the efficacy and mechanism of Zhizhu Granules(枳术颗粒)on functional dyspepsia(FD)rats.Methods A composite factor modeling method was used to induce FD model in rats.After successful modeling,rats were treated with Zhizhu Granules for two weeks.The effects of Zhizhu Granules on gastric residual rate,small intestinal propulsion rate,serum gastrin(GAS),growth hormone releasing peptide(Ghrelin),calcitonin gene related peptide(CGRP)and somatostatin(SS)contents in FD rats were observed;Hematoxylin-eosin(HE)staining was used to observe the pathological changes of gastric antrum tissues and duodenal tissues.The mRNA expressions of GAS,Ghrelin,CGRP and SS in hypothalamus and gastric tissues,mRNA expressions ofβ1-adrenergic receptor(ADRB1),somatostatin receptor(SSTR),ghrelin-O-acetyltransferase(GOAT),growth hormone secreting hormone receptor(GHSR),c-kit protooncogene protein(C-kit),stem cell factor(SCF),anoctamin-1(ANO1),myosin light chain kinase(MLCK)in gastric tissues and duodenal tissues were detected by qRT-PCR;Western blotting was used to detect the protein expressions of C-kit and SCF in gastric tissues and duodenal tissues.Results Zhizhu Granules could significantly reduce the gastric residual rate(P<0.05),increase the small intestine propulsion rate(P<0.01),increase the contents of Ghrelin and GAS in serum(P<0.01),reduce the contents of CGRP and SS(P<0.05,0.01),increase the expression levels of Ghrelin and GAS mRNA in hypothalamus and gastric antrum tissues(P<0.05,0.01),decrease the expression levels of CGRP and SS mRNA in hypothalamus and gastric antrum tissues(P<0.01),significantly increase the expression levels of SCF and C-kit proteins in gastric tissues and duodenal tissues(P<0.05,0.01),increase the expression levels of ADRB1,GOAT,GHSR,SCF,C-kit,ANO1 and MLCK mRNA in gastric tissues and duodenal tissues(P<0.05,0.01),and reduce SSTR mRNA expression level(P<0.05,0.01).Conclusion Zhizhu Granules can improve gastric motility abnormalities in FD rats,and its mechanism may be related to regu

关 键 词：枳术颗粒 功能性消化不良 脑肠肽 CAJAL间质细胞 胃饥饿素 

分 类 号：R285.5[医药卫生—中药学]