过敏原对过敏性鼻炎患者外周血CD4^(+)Th1 IL-18、IL-18BPa和IL-18Rα表达的影响  

Effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rα in blood CD4^(+)Th1 cells of patients with allergic rhinitis

作　　者：王君灵 湛萌萌 谷芳秋 李翼飞 张钊龙 赵悰怡 赵丹阳 郑卉 张祎捷 秦秉玉 WANG Junling;ZHAN Mengmeng;GU Fangqiu;LI Yifei;ZHANG Zhaolong;ZHAO Congyi;ZHAO Danyang;ZHENG Hui;ZHANG Yijie;QIN Bingyu(Department of Critical Care Medicine,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou 450003,China;Department of Respiration,Huaihe Hospital of Henan University,Kaifeng 475000,China;Allergy and Clinical Immunology Research Centre,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China;不详)

机构地区：[1]郑州大学人民医院河南省人民医院重症医学部,郑州450003 [2]河南大学淮河医院呼吸内科,河南开封475000 [3]锦州医科大学附属第一医院变态反应与临床免疫研究中心,辽宁锦州121001

出　　处：《实用医学杂志》2024年第11期1513-1518,共6页The Journal of Practical Medicine

基　　金：河南省重点研发与推广专项项目(编号:232102311022);博士后科研启动基金项目(编号:HN2022097,ZC20230003);辽宁省自然科学基金项目(编号:2019-ZD-0801,LJKZ0796);锦州医科大学博泽青年科技人才托举基金项目(编号:JYBZQT2110)。

摘　　要：目的探讨过敏原对健康人(healthy control,HC)和过敏性鼻炎(allergic rhinitis,AR)患者外周血CD4^(+)Th1细胞表达IL-18、IL-18BPa和IL-18Rα蛋白水平及mRNA水平的影响。方法收集皮肤点刺试验阴性的鼻炎(AR^(-))、皮肤点刺试验阳性的鼻炎(AR^(+))患者和HC的外周血标本。流式细胞术检测过敏原对CD4^(+)Th1细胞表达IL-18、IL-18BPa和IL-18Rα蛋白水平的影响;qPCR检测过敏原对CD4^(+)T细胞表达IL-18、IL-18BPa和IL-18RαmRNA水平的影响。结果与HC相比,AR^(-)和AR^(+)患者外周血Th1细胞中IL-18蛋白的表达增加,IL-18BPa蛋白的表达减少;AR^(+)患者外周血Th1细胞中IL-18Rα蛋白的表达增加;过敏原可增强HC Th1细胞IL-18Rα蛋白的表达,并诱导AR^(+)患者和HC CD4^(+)T细胞IL-18、IL-18BPa和IL-18RαmRNA的表达。结论过敏原极可能通过促进外周血CD4^(+)Th1细胞表达IL-18和IL-18Rα进而参与AR发病。Objective To investigate the effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rαprotein in peripheral blood CD4^(+)Th1 cells of healthy control subjects(HC)and patients with allergic rhinitis(AR),and on the expressions of IL-18,IL-18BPa and IL-18Rα mRNA in the peripheral blood CD4^(+)T cells.Methods Blood samples were collected from patients with rhinitis for negative skin prick test(AR^(-)),rhinitis for positive skin prick test(AR^(+))and HC.Flow cytometry was used to evaluate the effects of allergens on the expressions of IL-18,IL-18BPa and IL-18Rαprotein in CD4^(+)Th1 cells.The expressions of IL-18,IL-18BPa and IL-18RαmRNA in CD4^(+)T cells were determined by qPCR.Results Compared with HC,increased IL-18 while decreased IL-18BPa expressions in Th1 cells of AR^(-)and AR^(+)patients were observed,increased IL-18Rαexpression in Th1 cells of AR^(+)patients was also found.Additionally,allergens induced elevated expression of IL-18Rα protein in Th1 cells of HC,and induced elevated mRNA expressions of IL-18,IL-18BPa and IL-18Rα in isolated blood CD4^(+)T cells of AR^(+)patients and HC.Conclusion Allergens may be involved in the pathogenesis of AR by inducing the expressions of IL-18 and IL-18Rαin blood CD4^(+)Th1 cells.

关 键 词：过敏性鼻炎 CD4阳性Th1细胞 白细胞介素-18 白细胞介素-18受体α 

分 类 号：R392.8[医药卫生—免疫学]