不同品种楠木提取物抗炎活性评价  

Evaluation of Anti-Inflammatory Activity of Extracts from Different Species of Kusunoki

作　　者：伍艳芳[1] 谢传奇 卢桐溪 吴磊[2] WU Yanfang;XIE Chuanqi;LU Tongxi;WU Lei(Camphor Engineering Technology Research Center for National Forestry and Grassland Administration,Jiangxi Academy of Forestry,Nanchang 330013,China;Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330096,China;School of Environmental Science and Engineering,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]江西省林业科学院国家林业和草原局樟树工程技术研究中心,江西南昌330013 [2]江西省科学院应用化学研究所,江西南昌330096 [3]浙江工商大学环境科学与工程学院,浙江杭州310018

出　　处：《生物化工》2024年第2期39-46,共8页Biological Chemical Engineering

基　　金：江西省林业局林业科技创新专项((CXZX)(2021)15);江西省林业科学院基础研究与人才科研专项项目(2023522703);江西省科学院省级包干制一般项目(2023YSBG22026)。

摘　　要：本研究对5种楠木的抗炎活性进行了初步评价,以期为进一步开发楠木资源和相关产品提供研究基础。采用溶剂萃取方式,对粗壮润楠、广东润楠、柳叶润楠、浙江润楠和闽楠等5种楠木进行提取,并以脂多糖诱导的RAW264.7细胞为炎症模型,采用Griess法和MTT法分别评价不同提取物对NO产生和细胞存活率的影响。结果表明,粗壮润楠二氯甲烷和乙酸乙酯提取物、广东润楠正己烷提取物、柳叶润楠正己烷提取物、浙江润楠二氯甲烷提取物、闽楠水提取物对脂多糖诱导的RAW264.7细胞NO产生具有较好抑制作用,且对细胞存活率影响较小,其中粗壮润楠二氯甲烷提取物对NO的抑制作用最强。粗壮润楠二氯甲烷提取物的抗炎活性最好,有望进一步开发研究。The anti-inflammatory activities of 5 species of kusunoki are preliminarily evaluated to provide research basis for further development of kusunoki resources and related products.Five species of kusunoki,including Machilus robusta,Machilus kwangtungensis,Machilus salicina,Machilus chekiangensis and Phoebe bournei are extracted by solvent extraction,RAW264.7 cells induced by lipopolysaccharide(LPS)are used as inflammation model,NO production is evaluated by Griess method,and cell viability is evaluated by MTT method.The results show that the extraction of dichloromethane and ethyl acetate from Machilus robusta,extraction of n-hexane from Machilus kwangtungensis,extraction of n-hexane from Machilus salicina,extraction of dichloromethane from Machilus chekiangensis and extraction of water from Phoebe bournei have a good inhibitory effect on the production of NO in LPS-induces RAW264.7 cells,and have little effect on cell viability.Among them,extraction of dichloromethane from Machilus robusta had the strongest inhibitory effect on NO production.The extraction of dichloromethane from Machilus robusta has the best anti-inflammatory activity,which is worthy of further investigation and development.

关 键 词：楠木 溶剂萃取 抗炎 NO产生 细胞存活率 

分 类 号：Q946.8[生物学—植物学]