赤桉蜂蜜对小鼠酒精性肝损伤的保护作用  

Protective Effect of Jarrah Honey Against Acute Alcoholic Liver Injury in Mice

作　　者：王靖云 林韦康 陶春霖 WANG Jingyun;LIN Weikang;TAO Chunlin(Wang Shuhe(Zhengzhou)Biopharmaceutical Engineering Co.,Ltd.,Zhengzhou 450000,China;Wang Shuhe Biomedical(Wuhan)Co.,Ltd.,Wuhan 430040,China;Yangzhuoyuan(Shanghai)Network Technology Co.,Ltd.,Shanghai 201100,China)

机构地区：[1]王叔和(郑州)生物制药工程有限公司,河南郑州450000 [2]王叔和生物医药(武汉)有限公司,湖北武汉430040 [3]洋妆源(上海)网络科技有限公司,上海201100

出　　处：《生物化工》2024年第2期111-115,共5页Biological Chemical Engineering

摘　　要：目的:探究赤桉蜂蜜(Jarrah Honey,JH)对小鼠酒精性肝损伤的保护作用。方法:将50只小鼠随机分为5组:空白对照组、模型组、阳性对照组(普通蜂蜜15 g/kg)以及JH低、高剂量组(5 g/kg、15 g/kg)。各组连续灌胃15 d,在第16~18 d,于末次给药1 h后,除对照组给予生理盐水外,其他组均给予15 mL/kg白酒,建立急性肝损伤模型。通过计算小鼠的肝脏指数,检测小鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性、肝脏抗氧化酶含量、炎症因子以及肝脏病理观察,综合评价JH对急性酒精性肝损伤的保护作用。结果:与空白对照组相比,模型组小鼠肝脏指数显著提高,血清中ALT、AST活性上升,肝脏抗氧化能力降低,出现炎症反应,肝脏细胞变性,表示模型建立成功;与模型组相比,JH低、高剂量组小鼠肝脏指数明显降低,血清中ALT、AST活性降低,肝脏中抗氧化酶过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平升高,炎症反应改善,肝脏组织破坏程度减轻。结论:JH对酒精性肝损伤具有一定的保护作用,且存在量效关系,当使用量为15 g/kg时,可以有效降低酒精对肝脏的伤害程度。Objective:To investigate the protective effect of Jarrah Honey(JH)on alcoholic liver injury in mice.Methods:Fifty mice are randomly divided into five groups:control group,model group,positive control group(common honey 15 g/kg)and JH low and high dose groups(5 g/kg,15 g/kg).After intragastric administration for 15 consecutive days,from the 16th to the 18th day,all mice except those in the blank control group are administered with alcohol at a dose of 15 mL/kg by gavage to establish an acute liver injury model.The protective effect of JH on acute alcoholic liver injury is comprehensively evaluated by calculating the liver index of mice,detecting the activity of alanine aminotransaminase(ALT)and aspartate aminotransferase(AST)in serum,the content of liver antioxidant enzymes,inflammatory factors and liver pathological observation.Results:Compared with blank control group,the liver index of the model group mice is significantly increased,the activities of ALT and AST in serum are increased,the antioxidant capacity of the liver is reduced,inflammatory reactions occurred,and liver cell degeneration occurred,indicating the successful establishment of the model;compared with the model group,the liver index of mice in the low and high dose JH groups is significantly reduced,and the activities of ALT and AST in serum are reduced.The levels of antioxidant enzymes catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the liver are increased,and the inflammatory response is improved.The degree of liver tissue damage is also reduced.Conclusion:JH has a certain protective effect on alcoholic liver injury,and there is a dose effect relationship.When the dosage is 15 g/kg,JH can effectively reduce the degree of alcoholic liver injury.

关 键 词：赤桉蜂蜜 小鼠 酒精 肝损伤 

分 类 号：S896.6[农业科学—特种经济动物饲养]