固定床式生物反应器在293T细胞高密度培养中的应用  

Application of Packed-Bed Bioreactor on High Density of 293T Cell Culture

作　　者：曹灿 汪兴 张君轩 程黄鹤 CAO Can;WANG Xing;ZHANG Junxuan;CHENG Huanghe(T&J Bio-Engineering(Shanghai)Co.,Ltd.,Shanghai 201613,China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036 China)

机构地区：[1]迪必尔生物工程(上海)有限公司,上海201613 [2]安徽农业大学动物科技学院,安徽合肥230036

出　　处：《生物化工》2024年第2期154-157,共4页Biological Chemical Engineering

基　　金：国家重点研发计划(2021YFC2103300)。

摘　　要：目的:研究悬浮和贴壁293T细胞在生物反应器内的高密度培养工艺。方法:分别在2个3.5 L固定床生物反应器中加入150 g片状载体,接种贴壁293T细胞和驯化为悬浮的293T细胞,根据葡萄糖消耗情况调整灌流量进行连续灌流培养6天。结果:贴壁293T细胞和悬浮293T细胞均能在固定床生物反应器片状载体上生长,细胞密度最终分别达到2.5×10^(7)cells/mL和2.3×10^(7)cells/mL,6天增殖约50倍。结论:固定床式生物反应可以用于贴壁和悬浮293T细胞的高密度培养。Objective:To investigate the process for high-density cell culture of 293T cells in packed-bed bioreactor.Methods:The adherent 293T cells and suspending 293T cells are inoculated into two 3.5 L packed-bed bioreactors filled with 150 g Fibra-Cel disks.Adjust the perfusion volume according to glucose consumption and perform continuous perfusion culture for 6 days.Results:Both of adherent 293T cells and suspending 293T cells could grow in packed-bed bioreactors Fibra-Cel disks.In the end,the density of cells reached at 2.5×10^(7)cells/mL and 2.3×10^(7)cells/mL for adherent 293T cells and suspending 293T cells,respectively,with approximately increasing 50 folds in six days.Conclusion:The packed-bed bioreactor can be used for high density culture of adherent and suspending 293T cells.

关 键 词：生物反应器 293T细胞 连续灌流 片状载体 

分 类 号：Q813.11[生物学—生物工程]