单纯紫外线照射法建立黄褐斑小鼠模型研究  

Establishment of a mouse model of melasma using simple ultraviolet irradiation

作　　者：李丽颖 孙靖涵 华骋 陈振雨[1] 吕乐乐 杜宪 Li Liying;Sun Jinghan;Hua Cheng;Chen Zhenyu;Lyu Lele;Du Xian(Department of Aesthetic Plastic Surgery,Affiliated Hospital of Qingdao University,Qingdao 266003,China;Department of Burns and Plastic Surgery,Qingdao Central Hospital,Qingdao 266042,China;Beckman Laser Institute,Korea Dankook University College of Medicine,Cheonan 31116,Republic of Korea;Medical Aesthetic Centre,Qingdao New Century Women’s and Children’s Hospital,Qingdao 266011,China;Department of Burn,Qingdao Jun Liang Burn Hospital,Qingdao 266073,China)

机构地区：[1]青岛大学附属医院美容整形外科,青岛266003 [2]青岛市中心医院烧伤整形外科,青岛266042 [3]檀国大学医学院韩国贝克曼激光研究所,韩国天安31116 [4]青岛新世纪妇儿医院医学美容中心,青岛266011 [5]青岛君良烧伤医院烧伤科,青岛266073

出　　处：《中华整形外科杂志》2024年第4期444-454,共11页Chinese Journal of Plastic Surgery

基　　金：青岛大学附属医院医学伦理委员会批准(QYFYWZLL27859)。

摘　　要：目的探索单纯紫外线照射法建立黄褐斑小鼠模型的适宜照射能量及频率,为成功建立黄褐斑模型提供动物品系及照射方案参考。方法使用BALB/c雌鼠及C57BL/6JNifdc雌鼠建立黄褐斑动物模型,采用简单随机方法,将BALB/c雌鼠分为A、B、C、G 4组,每组各5只;将C57BL/6JNifdc雌鼠分为D、E、F、H 4组,每组各5只。所有小鼠均采用8.428 mW/cm^(2)紫外光予以照射,A、D组照射时间为15 s(单次照射能量为0.13 J/cm^(2)),B、E组照射时间为15 min(单次照射能量为7.59 J/cm^(2)),C、F组照射时间为30 min(单次照射能量为15.17 J/cm^(2)),连续5 d照射后停止2 d为1个周期,共照射4个周期;G、H组不予照射。照射结束后,所有小鼠正常饲养,并于1周后取材(每组3只),行HE、Masson-Fontana、Masson、免疫组化染色,分别测定棘细胞层厚度、黑色素颗粒数、胶原百分比、白细胞介素-1(IL-1)水平。其余小鼠继续饲养1周,脱毛并拍照,观察色斑变化。使用SPSS 27.0软件进行数据分析,不符合正态分布的计量资料采用M(Q 1,Q 3)表示,组间比较采用Kruskal-Wallis秩和检验。结果在全程照射过程中,BALB/c雌鼠各组始终未形成肉眼可见的色斑,而在C57BL/6JNifdc雌鼠中,第2周照射后D、E、F组均产生不同大小的色斑,然而第3周照射后,D组色斑逐渐消失,E组色斑则较前更加明显,与此同时,F组也产生了明显的色斑和部分小鼠背部皮肤出现脱屑甚至灼伤破损。第4周照射后,D组无新色斑形成,E组色斑更为明显,F组大部分出现皮肤灼伤破损。照射结束后2周,各组BALB/c雌鼠背部皮肤无明显色斑,而在C57BL/6JNifdc雌鼠中,D组无明显色斑,E组色斑颜色较第4周照射后变浅,F组皮肤灼伤破损处结痂,色斑较前变浅,但E、F组色斑仍明显肉眼可见。HE染色显示,A、B、C、G组棘细胞层厚度差异无统计学意义(H=1.08,P=0.782);而D、E、F、H组棘细胞层厚度差异有统计学意义(H=12.85,P=0.005),随着�ObjectiveTo determine the optimal irradiation energy and frequency for the establishment of melasma mouse model using simple ultraviolet irradiation,and to provide guidance on animal strains and irradiation protocols for the successful establishment of melasma model.MethodsAnimal models of melasma were established using BALB/c female mice and C57BL/6JNifdc female mice.BALB/c female mice were divided into 4 groups using a simple randomization method:A,B,C and G,with 5 mice in each group.C57BL/6JNifdc female mice were divided into 4 groups:D,E,F and H,with 5 mice in each group.All mice were irradiated with 8.428 mW/cm^(2)of ultraviolet light.The irradiation time was 15 s(single irradiation energy of 0.13 J/cm^(2))in groups A and D,15 min(single irradiation energy of 7.59 J/cm^(2))in groups B and E,and 30 min(single irradiation energy of 15.17 J/cm^(2))in groups C and F.Each cycle consisted of 5 consecutive days of irradiation followed by 2 days of cessation,totaling 4 cycles of irradiation.Groups G and H were not irradiated.At the end of irradiation,all mice were kept under normal conditions.One week later,3 mice from each group were selected for HE,Masson-Fontana,Masson,and immunohistochemical staining.Quantitative analysis was performed to measure the thickness of the acanthocyte layer,melanin granules,collagen percentage,and interleukin-1(IL-1)levels.The remaining mice were kept for an additional week,depilated and photographed to observe the changes in coloration.Data were analyzed using SPSS 27.0 software,measurement data that did not conform to normal distribution were represented by M(Q 1,Q 3)and comparisons between groups were made using the Kruskal-Wallis rank sum test.ResultsDuring the entire irradiation process,no visible discoloration was observed in the BALB/c female mice in all groups.In contrast,varying sizes of discoloration appeared in the C57BL/6JNifdc female mice in groups D,E,and F after irradiation in the second week.However,by the third week,the discoloration in group D gradually disappeared,w

关 键 词：晒斑 晒伤 黄褐斑 动物模型 紫外线照射法 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]