P19激活GPR56的Gq通路促进米色脂肪棕色化  

P19 activates the Gq pathway of GPR56 to promote browning of beige adipose

作　　者：王腾威 林慧 张明祥 孙金鹏[2] 张道来[1] WANG Tengwei;LIN Hui;ZHANG Mingxiang;SUN Jinpeng;ZHANG Daolai(School of Pharmacy,Binzhou Medical University,Yantai 264003,Shandong,China;Department of Basic Medical Sciences,Shandong University,Jinan 250012,Shandong,China)

机构地区：[1]滨州医学院药学院,山东烟台264003 [2]山东大学基础医学院,山东济南250012

出　　处：《山东大学学报（医学版）》2024年第3期20-27,共8页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(32130055,82371629,82304583)。

摘　　要：目的探究GPR56的Stachel peptide(P19)对米色脂肪组织棕色化的影响及其分子机制。方法利用qPCR和Western blotting方法检测GPR56在米色脂肪组织中的表达水平。用ELISA方法检测50μmol/L P19激活小鼠米色脂肪组织中GPR56的Gq通路而导致的下游3-磷酸肌醇(inositol triphosphate,IP3)水平变化。随后,在诱导原代米色脂肪细胞棕色化分化过程中,通过qPCR方法检测棕色化标志基因和产热标志基因的表达水平,对P19、YM-254890(YM)和沉默Gpr56基因的表达对分化进程的影响进行综合分析。结果GPR56在米色脂肪组织中高表达。P19可以使棕色化标志基因(Tbx和Tmem26)和产热标志基因(Ppargc1a、Cox7a1和Cidea)表达水平升高,差异有统计学意义(P<0.05)。此外,当加入YM或沉默Gpr56基因的表达时,这些棕色化标志基因和产热标志基因的表达水平出现了反转。结论P19能够通过激活GPR56的Gq通路来促进米色脂肪组织的棕色化过程。Objective To investigate the influence of GPR56 Stachel peptide(P19)on the browning of beige adipose tissue and its molecular mechanisms.Methods qPCR and Western blotting techniques were used to assess the expression levels of GPR56 in beige adipose tissue.ELISA assay was used to investigate the impact of 50μmol/L P19 on the downstream IP_(3)level changes caused by activation of GPR56 Gq pathway in mouse beige adipose tissue.Subsequently,during the induction of browning differentiation in primary beige adipocytes,the expression levels of browning marker genes and thermogenic marker genes were evaluated using qPCR.A comprehensive analysis was performed to elucidate the effects of P19,YM-254890(YM),and Gpr56 gene silencing on the differentiation process.Results GPR56 was highly expressed in beige adipose tissue.P19 can increase the expression levels of browning marker genes(Tbx and Tmem26)and heat production marker genes(Ppargc1a,Cox7a1 and Cidea)with statistically significant differences(P<0.05).Moreover,when YM was added or Gpr56 expression was silenced,a reversal in the expression levels of these browning and thermogenic marker genes was observed.Conclusion P19 can promote the browning process of beige adipose tissue through the activation of the Gq pathway.

关 键 词：GPR56 P19 黏附类G蛋白偶联受体 信号转导通路 脂肪分化 

分 类 号：Q54[生物学—生物化学]