对乙酰氨基酚引起的急性肝损伤对大鼠脑内内源性物质的影响  

Effects of Acetaminophen-induced Acute Liver Injury on Endogenous Substances in Rat Brain

作　　者：章嘉欣 叶熊龙 黄郅戬 武晓悦 程宏伟 孔维敏 ZHANG Jiaxin;YE Xionglong;HUANG Zhijian;WU Xiaoyue;CHENG Hongwei;KONG Weimin(Department of Pharmacy,Hunan Provincial People′s Hospital/the First Hospital Affiliated to Hunan Normal Univer-sity,Changsha,Hunan Province,410002 China;School of Pharmacy,Bengbu Medical University,Bengbu,Anhui Province,233030 China)

机构地区：[1]湖南省人民医院/湖南师范大学附属第一医院药学部,湖南长沙410002 [2]蚌埠医科大学药学院,安徽蚌埠233030

出　　处：《系统医学》2024年第8期36-39,共4页Systems Medicine

基　　金：2022年安徽省高等学校科学研究项目(自然科学类)(2022AH051459)。

摘　　要：目的探索对乙酰氨基酚引起的急性肝损伤对大鼠脑内内源性物质的影响。方法2023年4—6月在蚌埠医科大学选取12只雄性Sprague-Dawley大鼠,采用随机数表法分为对照组(生理盐水灌胃)和对乙酰氨基酚组(以灌胃给药方式建立急性肝损伤模型),各6只。测定大鼠肝脏谷丙转氨酶和谷草转氨酶活力,脑内多巴胺和5-羟色胺含量及血浆中5-羟色胺含量。结果对乙酰氨基酚组大鼠肝脏谷丙转氨酶和谷草转氨酶活力均显著高于对照组[(30.31±18.02)U/L vs(7.58±1.65)U/L],[(53.53±26.54)U/L vs(16.99±1.35)U/L],差异有统计学意义(t=-2.887、-2.882,P均<0.01)。与对照组相比,对乙酰氨基酚组大鼠的皮层和海马组织多巴胺含量内分别增加了9.9%和8.6%,在皮层、海马组织和血浆内5-羟色胺含量分别增加了14.8%、3.1%和9.5%,但差异无统计学意义(P均>0.05)。结论与对照组相比,对乙酰氨基酚引起的急性肝损伤使大鼠脑内的内源性物质水平有上升趋势,但差异无统计学意义。造成这种结果的原因可能是对乙酰氨基酚给药剂量或给药频率不够所致的肝损伤程度不够。Objective To explore the effects of acetaminophen-induced acute liver injury on endogenous substances in rat brain.Methods Twelve male Sprague-Dawley rats were selected from April to June 2023 at Bengbu Medical University and divided into six each of control group(physiological saline gavage)and acetaminophen group(to establish an acute liver injury model by gavage administration)by random number table method.The liver alanine aminotransferase and aspartate aminotransferase activities,brain dopamine and 5-hydroxytryptamine levels and plasma 5-hydroxytryptamine levels were measured in rats.Results The liver alanine aminotransferase and aspartate aminotransferase activities of rats in the acetaminophen group were significantly higher than those of the control group[(30.31±18.02)U/L vs(7.58±1.65)U/L],[(53.53±26.54)U/L vs(16.99±1.35)U/L],and the differences were statistically significant(t=-2.887,-2.882,both P<0.01).Compared with the control group,rats in the acetaminophen group showed an increase of 9.9%and 8.6%within the cortical and hippocampal tissue dopamine content,respectively,and 14.8%,3.1%,and 9.5%within the cortical,hippocampal tissue,and plasma 5-hydroxytryptophan content,respectively,but the difference was not statistically significant(all P>0.05).Conclusion Acute liver injury induced by acetaminophen resulted in a trend toward increased levels of endogenous substances in the rat brain compared with the control group,but there was no statistically significant difference.It is hypothesized that the reason for this result may be the insufficient level of liver injury due to the insufficient dose or frequency of acetaminophen administration.

关 键 词：对乙酰氨基酚 急性肝损伤 多巴胺 5-羟色胺 

分 类 号：R9[医药卫生—药学]