那他霉素在新西兰白兔眼组织中的浓度测定及眼组织分布考察  

Investigation on Concentration Determination and Tissue Distribution of Natamycin in New Zealand White Rabbit Eyes

作　　者：孙璐瑶 缪峰 季金风 宋鑫 赵利媛 邱云良 SUN Luyao;MIAO Feng;JI Jinfeng;SONG Xin;ZHAO Liyuan;QIU Yunliang(School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230012;InnoStar Biotechnology Nantong Co.,Ltd.,Nantong 226133;Yangtze Delta Drug Advanced Research Institute,Nantong 226133;Shanghai Innostar Biotech Co.,Ltd.,Shanghai 201203)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]益诺思生物技术南通有限公司,江苏南通226133 [3]长三角药物高等研究院,江苏南通226133 [4]上海益诺思生物技术股份有限公司,上海201203

出　　处：《中国医药工业杂志》2024年第4期533-539,共7页Chinese Journal of Pharmaceuticals

摘　　要：该研究建立了那他霉素(1)的LC-MS/MS分析方法,并测量了1在新西兰白兔眼部不同组织中的分布情况。结果显示,新西兰白兔经玻璃体腔注射给予1后,1在眼组织中广泛分布,且更多分布在眼后节。1在视网膜和玻璃体中的c_(max)(分别为46400和4620 ng/g)、AUC_(0→24)(分别为232000和29300 h·ng·g^(-1))均高于其他眼组织。给药24 h后,1在眼部各组织中的浓度依然较高,提示其保留时间较长。该研究建立的LC-MS/MS方法专属性高,结果可靠,可用于1的眼组织分布研究,为拓宽1用于眼部疾病的治疗提供了思路。In this study,a LC-MS/MS analytical method was developed for determination of natamycin(1)to assess its distribution in various ocular tissues of New Zealand white rabbits.The results revealed that,after intravitreal administration of 1 to New Zealand white rabbits,1 was widely distributed in various ocular tissues,particularly in the posterior segment of the eye.The c_(max )and AUC_(0→24) of 1 in retina and vitreous were 46400 and 4620 ng/g,232000 and 29300 h·ng·g^(–1),respectively.The results demonstrated that the 1 amounts were higher in both retina and vitreous than in other ocular tissues.After 24 hours post-dosing,the drug concentration remained high level in all ocular tissues,indicating a longer retention of 1.Given this high specificity and reliable results,this established LC-MS/MS method is suitable for investigating distribution of 1 in ocular tissues and can be used to explore further potential treatments for ocular diseases with 1.

关 键 词：那他霉素 LC-MS/MS 定量分析 组织分布 玻璃体腔注射 

分 类 号：R978.5[医药卫生—药品] R917[医药卫生—药学]