育阴灵加减方对D-半乳糖所致POI模型大鼠Hippo信号通路的影响  

Effect of Modified Yuyinling Formula on Hippo Signaling Pathway in POI Model Rats Induced by D-galactose

作　　者：冯聪[1] 齐娜[2] 冯涛[3] 李琛[1] 张跃辉[1] 王逸 白茸 FENG Cong;QI Na;FENG Tao;LI Chen;ZHANG Yuehui;WANG Yi;BAI Rong(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang Provincial Hospital,Harbin 150036,China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040 [3]黑龙江省医院,黑龙江哈尔滨150036

出　　处：《中医药学报》2024年第6期31-37,共7页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金联合引导项目(LH2022H079);黑龙江省博士后特别资助项目(LBH-TZ2217);黑龙江省博士后资助面上项目(LBH-Z21079);2023黑龙江省中医药科研项目(ZHY2023-084)。

摘　　要：目的:研究育阴灵加减方对早发性卵巢功能不全(POI)模型大鼠的影响及其可能作用机制。方法:35%D-半乳糖造模成功的POI模型子代雌性大鼠40只随机分为模型组,育阴灵加减方高(0.562 g/kg)、中(0.281 g/kg)、低(0.141 g/kg)剂量组和西药组,西药组给予戊酸雌二醇溶液0.120 mg/kg,第4天加灌醋酸甲轻孕酮片溶液0.960 mg/kg,随后两药停用1 d,为1个疗程,灌胃4个疗程。未造模的8只子代雌性大鼠作为空白组。观察大鼠体质量、卵巢及子宫系数、卵巢组织的病理变化、血清抗缪勒管激素(AMH)、抑制素B(INHB)、血管内皮生长因子(VEGF)水平,蛋白免疫印迹法及荧光定量PCR法测定卵巢组织中Hippo信号通路相关效应因子表达情况。结果:与模型组相比,育阴灵加减方高、中剂量组可以升高大鼠体质量、卵巢系数、子宫系数(P<0.05),可改善卵巢组织结构并升高大鼠血清AMH、INHB、VEGF水平(P<0.05),两组Hippo信号通路相关效应因子Mst1、Lats2、YAP1、p-YAP、Smad7的表达量明显降低(P<0.05)。结论:育阴灵加减方可能通过调控Hippo信号通路的表达改善POI模型大鼠的卵巢功能。Objective:To study the effect of modified Yuyinling formula(YYLF)on POI model rats and its possible mechanism.Methods:The POI rat model was established by 35%D-galactose,40 female rats were randomly divided into model group,Chinese medicine high-dose(0.562 g/kg),medium-dose(0.281g/kg),and low-dose(0.141g/kg)groups,and Western medicine group.The Western medicine group was gavaged diethylstilbestrol solution at a dose of 0.120 mg/kg,and on the fourth day,mifepristone solution at a dose of 0.960 mg/kg was gavaged,and then,both drugs were discontinued for 1 day,as a treatment course,and four treatment courses were carried out.Eight non-modeled female rats were used as the blank group.Observations were made on the body weight of rats,ovarian and uterine coefficients,pathological changes in ovarian tissue,serum levels of anti-Müllerian hormone(AMH),inhibin B(INHB),and vascular endothelial growth factor(VEGF).The expression of Hippo signaling pathway-related effector factors in ovarian tissue were detected using protein immunoblotting and fluorescence quantitative PCR.Results:Compared with model group,body weight,ovarian coefficient and uterus coefficient of rats in high and medium dose group of YYLF were raised(P<0.05);YYLF could improve ovarian tissue structure,could raise the serum AMH,INHB and VEGF levels(P<0.05),and could reduce the expression levels of Hippo signaling pathway related effect factors Mst1,Lats2,YAP1,p-YAP and Smad7.Conclusion:Modified Yuyinling formula may improve the ovarian function of POI model rats by regulating the expression of Hippo signaling pathway.

关 键 词：早发性卵巢功能不全 育阴灵加减方 卵巢结构 Hippo信号通路 

分 类 号：R285.5[医药卫生—中药学]