贵阳地区血小板捐献者HPA-1~6/10/15/21和HLA-A/B基因多态性研究  

Research of HPA-1-6/10/15/21 and HLA-A/B gene polymorphism in platelet donors of Guiyang

作　　者：王军[1] 侯仕芳 王娇[1] 雷雨 余娜杰 赵霞 朱思刚 WANG Jun;HOU Shifang;WANG Jiao;LEI Yu;YU Najie;ZHAO Xia;ZHU Sigang(Blood Transfusion Research Laboratory,Guizhou Provincial Blood Center,Guiyang 550003,Guizhou,China)

机构地区：[1]贵州省血液中心输血研究室,贵州贵阳550003

出　　处：《贵州医科大学学报》2024年第5期686-690,共5页Journal of Guizhou Medical University

基　　金：2022年贵州省卫生健康委技术基金项目(gzwkj2022-178)。

摘　　要：目的研究贵阳地区机采血小板捐献者人类血小板抗原HPA-1~6/10/15/21及人类白细胞抗原HLA-A、B基因分布及多态性。方法采用实时荧光定量PCR法(qPCR)对287名贵阳地区机采血小板捐献者进行HPA-1~6/10/15/21和HLA-A、B基因分型,列举aa\ab\bb基因及HLA-A、B等位基因的分布情况、计算aa\ab\bb基因及HLA-A、B等位基因型频率。结果287名血小板捐献者HPA-1~6/10/15/21系统中,HPA-4和HPA-10的基因型均为aa型,不具有多态性;HPA-1,2,5,6和21主要以aa型为主;仅在HPA-3和HPA-15中检出bb型;杂合度最高的是HPA-15,HPA3杂合度居次;HLA-A位点检出14个等位基因,频率最高的3个是A*02(0.36)、A*11(0.33)和A*24(0.162;HLA-B位点检出23个等位基因,频率最高的4个是B*46(0.19)、B*15(0.149、B*40(0.14)和B*13(0.13)。结论贵阳地区机采血小板捐献者HPA-1~6/10/15/21和HLA-A、B基因存在多态性,需建立该地HPA/HLA基因分型血小板供者库服务于临床。Objective To study the gene distribution and polymorphism of human platelet antigen HPA-1-6/10/15/21 and human leukocyte antigens HLA-A and B from human-collected platelet donors in Guiyang.Methods The real-time fluorescent quantitative PCR method(qPCR)on 287 guiyang region machine adopt platelet donors in HPA-1-6/10/15/21 and HLA-A,B genotyping,The distribution of aa\ab\bb gene and HLA-A and B alleles were listed,and the frequencies of aa\ab\bb gene and HLA-A and B alleles were calculated.Results In the HPA-1-6/10/15/21 system of 287 platelet donors,the genotypes of HPA-4 and HPA-10 were both aa type and were not polymorphic.HPA-1,2,5,6 and 21 are mainly aa type,and the bb-type was detected only in HPA-3 and HPA-15.The highest heterozygosity was found in HPA-15,followed by the second HPA 3 heterozygosity.14 alleles were detected at the HLA-A site,and the three highest frequencies were A*02(0.36),A*11(0.33),and A*24(0.162).Twenty-three alleles were detected at the HLA-B locus,and the four highest frequencies were B*46(0.19),B*15(0.149),B*40(0.14),and B*13(0.13).Conclusion There are polymorphisms in HPA-1-6/10/15/21 and HLA-A and B genes of platelet donors in Guiyang,so it is necessary to establish a local HPA/HLA genotyping platelet donor bank for clinical use.

关 键 词：血小板 人类血小板抗原 人类白细胞抗原 HPA/HLA基因 多态性 基因频率 

分 类 号：R331.1[医药卫生—人体生理学] R329.2[医药卫生—基础医学]