橘皮素缓解APP/PS1小鼠小胶质细胞过度激活及认知功能障碍  

Tangeretin Alleviates Microglia Over-activation and Cognitive Impairment in APP/PS1 Mice

作　　者：于晶 梁征 贺子珊 陈鸿霖 石成英 龚晓康 包建 舒细记 YU Jing;LIANG Zheng;HE Zishan;CHEN Honglin;SHI Chengying;GONG Xiaokang;BAO Jian;SHU Xiji(Wuhan Institute of Biomedical Sciences,Jianghan University,Wuhan 430056,Hubei,China)

机构地区：[1]江汉大学武汉生物医学研究院,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2024年第3期44-50,共7页Journal of Jianghan University：Natural Science Edition

基　　金：湖北省自然科学基金面上项目(2022CFB456)。

摘　　要：目的观察橘皮素(tangeretin,TAN)对APP/PS1小鼠认知功能,以及脑内小胶质细胞过度激活和炎症因子分泌的影响。方法2.5月龄雄性APP/PS1小鼠,通过口服给予TAN,连续干预16周,观察TAN对缓解APP/PS1认知功能障碍,以及脑内小胶质细胞过度激活和炎症因子分泌的作用。结果通过行为学检测发现TAN明显改善APP/PS1小鼠认知障碍;通过免疫荧光染色,发现TAN显著缓解APP/PS1小鼠脑内小胶质细胞的过度激活;多因子分析检测发现,TAN显著升高小鼠脑内抑炎因子IL-2和IL-5的水平。结论TAN显著减轻APP/PS1小鼠脑内小胶质细胞过度激活以及调节炎症因子分泌,缓解小鼠的认知障碍。Objective To study the effects of tangeretin(TAN)on cognitive performance,microglia over-activation,and inflammatory factors released in APP/PS1 mice.Methods 2.5-month-old male APP/PS1 mice were treated with TAN orally for 16 weeks.The effects of TAN on alleviating cognitive dysfunction of APP/PS1,microglia overactivation,and inflammatory factors released in the brain were observed.Results It was found that TAN significantly alleviated cognitive impairment in APP/PS1 mice by behavioral testing;the results of immunofluorescence staining demonstrated that TAN significantly alleviated microglia over-activation in the brain of APP/PS1 mice;the results of multifactor analysis showed that TAN significantly increased the levels of anti-inflammatory molecules IL-2 and IL-5 in the mouse brain.Conclusion TAN significantly reduces microglia over-activation,regulates the release of inflammatory factors in the brain of APP/PS1 mice,and alleviates cognitive impairment.

关 键 词：阿尔兹海默症 橘皮素 小胶质细胞 炎症因子 

分 类 号：R285.5[医药卫生—中药学] R749.16-33[医药卫生—中医学]