基于UPLC-Q-TOF-MS/MS技术的黄芩清热除痹胶囊化学成分分析  

Analysis of Chemical Constituents in Huangqin Qingre Chubi Capsule Based on UPLC-Q-TOF-MS/MS

作　　者：姜娟娟 刘晓闯[1] 张艳艳 张明媚 刘健[3] JIANG Juan-juan;LIU Xiao-chuang;ZHANG Yan-yan;ZHANG Ming-mei;LIU Jian(Department of Pharmacy,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Department of Rheumatology,The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China)

机构地区：[1]安徽中医药大学第一附属医院药学部,安徽合肥230031 [2]安徽中医药大学药学院,安徽合肥230012 [3]安徽中医药大学第一附属医院风湿科,安徽合肥230031

出　　处：《分析测试学报》2024年第6期814-825,共12页Journal of Instrumental Analysis

基　　金：安徽省高等学校科学研究项目(2023AH050861);中药制药过程新技术国家重点实验室项目(SKL2020Z0102)。

摘　　要：应用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF-MS/MS)法,对黄芩清热除痹胶囊(HQC)的化学成分进行快速鉴定,采用Water ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,以0.1%甲酸水-乙腈为流动相进行梯度洗脱,采用电喷雾电离源(ESI)在正、负离子模式下分别采集HQC的基峰色谱图。建立HQC的化学成分数据库,基于天然产物后处理筛查平台(UNIFI)进行靶向分析,结合化合物的精确分子量、二级碎片离子信息、对照品和文献数据等进行化学结构确认。从HQC中共鉴定出82个化合物,包括37个黄酮类、11个有机酸类、14个萜类、7个苷类、5个氨基酸类、8个其他类化合物;HQC主要以黄酮类成分为主。UPLC-Q-TOF-MS/MS结合UNIFI平台的分析方法可快速鉴定HQC的化学成分。An ultra-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)method was applied to identify chemical constituents rapidly of Huangqin Qingre Chubi Capsule(HQC).The gradient elution was performed on a Water ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)with 0.1%formic acid-acetonitrile as mobile phases.Positive and negative ion ESI modes were used simultaneously to collect base peak chromatograms of HQC.In addition,a self building library of HQC was established and targeted sceening based on UNIFI platform.The chemical constituents were identified and confirmed by analyzing the precise mo⁃lecular weight of the compound,secondary fragment ion information,reference products and litera⁃ture data.A total of 82 chemical constituents were tentatively identified,including 37 flavonoids,11 organic acids,14 terpenoids,7 glycosides,5 amino acids and 8 other compounds.HQC is mainly composed of flavonoids.The method of UPLC-Q-TOF-MS/MS combined with UNIFI can rap⁃idly identify the chemical components of HQC.

关 键 词：黄芩清热除痹胶囊 超高效液相色谱-四极杆-飞行时间质谱 UNIFI分析平台 化学成分 类风湿关节炎 

分 类 号：O657.7[理学—分析化学] R284.1[理学—化学]