小麦四个抗白粉病基因的KASP标记检测  

KASP Marker Detection of Four Powdery Mildew Resistant Genes in Wheat

作　　者：李玮[1] 宋国琦[1] 张迎迎 李玉莲[1] 张淑娟[1] 张荣志[1] 李吉虎 高洁[1] 李根英[1] Li Wei;Song Guoqi;Zhang Yingying;Li Yulian;Zhang Shujuan;Zhang Rongzhi;Li Jihu;Gao Jie;Li Genying(Crop Research Institute,Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in North Yellow&Huai River Valley,Ministry of Agriculture/National Engineering Laboratory for Wheat&Maize,Jinan 250100,China;College of Agricultural Science and Technology,Shandong Agriculture and Engineering University,Jinan 250100,China)

机构地区：[1]山东省农业科学院作物研究所/农业部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室,山东济南250100 [2]山东农业工程学院农业科技学院,山东济南250100

出　　处：《山东农业科学》2024年第5期36-41,共6页Shandong Agricultural Sciences

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2023A01);山东省重点研发计划项目(2019LZGC009,2022LZGCQY002)。

摘　　要：为提高Pm13的检测效率,了解Pm21、PmV、Pm12和Pm13共4个小麦抗白粉病基因在山东小麦育种中的利用情况,本研究通过测序和引物设计成功转化了Pm13的KASP标记,并采用4个基因的KASP标记对2022—2023年山东省小麦高产区试组和强筋区试组的140份参试品系和2份区试对照品种进行检测,结果显示140份材料均不含上述4个基因,表明它们在山东小麦育种中应用较少。本研究可为利用KASP标记开展小麦抗白粉病基因的分子标记辅助选择提供参考,促进其育种应用。In order to improve detection efficiency of Pm13,and to know the utilization status of four mildew resistant genes as Pm21,PmV,Pm12 and Pm13 in wheat breeding programs of Shandong Province,KASP marker of Pm13 was successfully transformed by PCR sequencing and primer designing.The KASP markers of the four genes were employed to detect 140 lines and 2 control cultivars of wheat in the 2022-2023 regional trails of Shandong,belonging to high yield group and high quality group.The result showed that none of the four genes were detected in any of the 140 materials,which indicated that these genes were rarely used in wheat breeding of Shandong Province.This study could provide references for KASP marker-assisted selec-tion of wheat powdery mildew resistant genes and promote their utilization in wheat breeding.

关 键 词：小麦 抗白粉病基因 Pm13 KASP标记 分子标记辅助选择 

分 类 号：S512.103.4[农业科学—作物学]