小菜蛾幼虫生防菌株毒力测定及产毒条件优化  

Virulence Determination and Optimization of Toxin Production Conditions of Biocontrol Strains of Plutella xylostella Larvae

作　　者：张梓月 芦俊佳[1] 杨明国 马云强[1] 田芸菁 勾朝金 刘勉 Zhang Ziyue;Lu Junjia;Yang Mingguo;Ma Yunqiang;Tian Yunjing;Gou Chaojin;Liu Mian(College of Landscape Architecture and Horticulture Sciences,Southwest Forestry University/Yunnan Provincial Key Laboratory of Forest Disaster Warning and Control,Kunming 650224,China)

机构地区：[1]西南林业大学园林园艺学院/云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《山东农业科学》2024年第5期132-137,共6页Shandong Agricultural Sciences

基　　金：云南省科研基金项目“十字花科蔬菜害虫小菜蛾生防菌株筛选及毒力测定”(2023Y0751);云南省农业联合专项“薇甘菊入侵监测数据融合方法与高精度预警模型研究”(202101BD070001-128);云南省“兴滇英才支持计划”青年人才项目(990123069);云南农业联合专项“粉红粘帚霉对楚雄腮扁叶蜂致病机理研究”(202301BD070001-091)。

摘　　要：为丰富小菜蛾(Plutella xylostella)生物防治的微生物资源,本研究选用粉红粘帚霉(Clonostachys rosea)、淡紫拟青霉(Purpureocillium lilacinum)和02号生赤壳属(Bionectria sp.)三种具有较好生防效果的菌株开展其对小菜蛾幼虫毒力的菌株筛选试验,同时对选出的高毒力菌株进行毒力测定和产毒条件优化。结果表明,三种菌株对小菜蛾幼虫都具有防治效果,其中以粉红粘帚霉的生防毒力最强。毒力测定显示,粉红粘帚霉的半致死时间(LT_(50))为30.88 h,半致死浓度(LC_(50))为4.365×10^(4)个孢子/mL。从产毒条件优化结果看出,粉红粘帚霉产毒的最适培养基为萨氏葡萄糖琼脂酵母膏(SDAY),最适pH值为6,最适培养时间为7 d。综上表明,粉红粘帚霉对小菜蛾幼虫具有毒力高、致病速度快等特点,具有较大的生防潜力,可作为防治小菜蛾的优良生防真菌。To enrich microbial resources for biological control of Plutella xylostella,this study was con-ducted using three fungi strains as Clonostachys rosea,Purpureocillium lilacinum and Bionectria sp.02 with good biocontrol effects.Their virulence to the larvae of P.xylostella was tested,and the strain with high viru-lence was selected,then its virulence was determined and the toxin production conditions were optimized.The results showed that the three strains were all effective in controlling the larvae of P.xylostella,and the most ef-fective one was C.srosea.The virulence test showed that the half lethal time(LT_(50))and half lethal concentra-tion(LC_(50))of C.rosea were 30.88 h and 4.365×10^(4) spores per milliliter.According to the optimization results of toxin production conditions of C.rosea,the optimal medium was SDAY,the optimal pH value was 6,and the optimal culture time was 7 days.In summary,C.rosea had the characteristics of high virulence and rapid pathogenicity to the larvae of P.xylostella,so it had great biocontrol potential and could be used as an excel-lent biocontrol fungus against P.xylostella.

关 键 词：小菜蛾幼虫 生防菌株筛选 毒力测定 粉红粘帚霉 产毒条件优化 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]