基于网络药理学验证褪黑素对急性肺损伤的抑制作用  

Validation of the Inhibitory Effect of Melatonin onAcute Lung Injury based on Network Pharmacology

作　　者：王碧红 李卓越 李秦汉 孙燕玲 吕建国[3] 林丽[2] WANG Bi-hong;LI Zhuo-yue;LIQin-han;SUNYan-ling;LV Jian-guo;LIN Li(School of Phamacy,Xianning Medical College,Hubei Universiy of Science and Technology Xianning,Hube,437100,China;School of Basic Medical Sciences,Xianning Medical College,Hubei University of Science and Technoogy;School of Clinical Medical Sciences,Xianning Medical Collge,Hubei University of Science and Technology)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院医学部基础医学院 [3]湖北科技学院医学部临床医学院

出　　处：《承德医学院学报》2024年第3期189-194,共6页Journal of Chengde Medical University

基　　金：湖北省大学生创新训练项目(S202110927022);湖北省大学生创新训练项目(S202210927035)。

摘　　要：目的本文旨在研究褪黑素(MT)对急性肺损伤(ALI)可能的干预靶点和其抑制作用。方法通过数据库筛选获取MT的主要靶点及ALI的疾病作用靶点。经过Venn图的解析MT和ALI的共同靶点。利用Cytoscape 3.8.2工具构建“药物-疾病”的网络模型。借助STRING工具对蛋白质之间的相互作用进行分析,并构造PPI网络。使用R工具进行GO和KEGG的富集分析。同时,构建ALI动物模型并进行MT治疗干预,观察肺组织病理学的变化,气管切开插管检测各组肺功能Ri-area、Re-area和Cldyn-area水平。结果经筛选后获得196个MT靶点、3948个ALI靶点及118个药物-疾病共同靶点。EGFR、HIF1A和ERBB2等均为MT靶向ALI疾病的关键核心点。在GO富集分析中,生物过程共涉及到1690种,细胞组分涉及到90种,而分子功能则涉及到176种。至于KEGG路径富集筛选中,共发现与此相关的157条生物通路。体内实验结果显示MT治疗干预对ALI有明显抑制作用。结论MT可能通过多靶点抑制ALI的发病和进展。Objective The purpose of this study was to investigate the possible intervention targets and inhibitory effects of melatonin(MT)on Acute lung injury(ALI).Methods The main targets of MT and the disease targets of ALI were obtained through database screening.The common targets of MT and ALI were analyzed by Venn diagram.The network model of“drug-disease”was built by Cytoscape 3.8.2.Protein interactions were analyzed by STRING and PPI networks were constructed.At the same time,an animal model of ALI was established and MT treatment was performed.The changes of lung histopathology were observed.The pulmonary function levels of Ri-area,Re-area and Cldyn-area in each group were detected by tracheotomy and intubation.Results 196 MT targets,3948 ALI targets and 118 drug-disease co-targets were obtained.EGFR,HIF1A and ERBB2 were the key core sites of MT targeting ALI disease.In the GO enrichment analysis,a total of 1690 biological processes,90 cellular components and 176 molecular functions were involved.As for KEGG pathway enrichment screening,a total of 157 biological pathways related to this were found.The results of in vivo experiments showed that MT therapeutic intervention had obvious inhibitory effect on ALI.Conclusion MT may inhibit the onset and progression of ALI through multiple targets.

关 键 词：褪黑素 急性肺损伤 网络药理学 

分 类 号：R563[医药卫生—呼吸系统]