基于GC-Q-Exactive-MS的灵芝孢子油化学成分定性分析  

Chemome Profiling of Ganoderma lucidum Spore Oil Using GC-Q-Exactive-MS

作　　者：李玮 彭博 吴念 李波 刘文静 宋月林 LI Wei;PENG Bo;WU Nian;LI Bo;LIU Wen-jing;SONG Yue-in(Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Amway(China)Botanical Research Center,Wuxi 214011,China;Amway(Shanghai)Innovation&Science Co.,Ltd.,Shanghai 201508,China;School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]北京中医药大学中药学院中药现代研究中心,北京100029 [2]安利(中国)植物研发中心,江苏无锡214011 [3]安利(上海)创新科技有限公司,上海201508 [4]河南中医药大学药学院,河南郑州450046

出　　处：《中国现代中药》2024年第5期799-806,共8页Modern Chinese Medicine

基　　金：青年拔尖人才支持计划项目。

摘　　要：目的:利用高分辨气相色谱-四级杆-静电轨道阱质谱法(GC-Q-Exactive-MS)定性分析灵芝孢子油的化学成分。方法:采用GC-Q-Exactive-MS,在电子轰击(EI)源下获取化合物的全扫描质谱数据,通过总结脂肪酸类、萜类、甾醇类及醛类化学成分的质谱裂解规律,并检索NIST、MassBank、HMDB、PubChem在线数据库及查阅相关文献,对灵芝孢子油的各类化学成分进行鉴定。结果:从灵芝孢子油中共鉴定了22个化学成分,包括3个脂肪酸类、4个萜类、4个甾醇类、4个醛类及7个其他类化合物。结论:研究结果为灵芝孢子油质量控制和中药弱极性化学成分定性分析提供了参考。Objective:A high-resolution gas chromatography-quadrupole-electrostatic orbital trap mass spectrometry(GC-Q-Exactive-MS)was established for qualitative analysis of chemical components of Ganoderma lucidum spore oil.Methods:The full scan mass spectrometry data of the compounds in the electron ionization(EI)mode were acquired using GC-Q-Exactive-MS,and the various components of G.lucidum spore oil were identified via matching those proposed mass fragmentation pathways of fatty acids,terpenes,sterols and aldehydes,and searching online databases such as NIST,MassBank,HMDB,PubChem,as well as reference information in the relevant literatures.Results:A total of 22 chemical components were identified from G.lucidum spore oil,including 3 fatty acids,4 terpenes,4 sterols,4 aldehydes and 7 other compounds via matching those proposed mass fragmentation rules of fatty acids,terpenes,sterols and aldehydes,along with available databases and reference information in the literatures.Conclusion:The chemical profiling of G.lucidum spore oil is analyzed comprehensively,which provides a research basis for its quality control,and provides a reference for the qualitative analysis of weakly polar chemical components in traditional Chinese medicine.

关 键 词：灵芝孢子油 气相色谱-四级杆-静电轨道阱质谱法 质谱裂解规律 化学成分 

分 类 号：R284[医药卫生—中药学]