miR-34c-5p靶向poFUT1对胎盘血管形成的影响  

Effect of miR-34c-5p targeting poFUT1 on placental angiogenesis

作　　者：刘月华[1] 杨照远 鲁继聪 谢梦霞 郭婧[1] 王媛媛[1] 杨敬敬[1] 田赟 赵明 陈冬笋[1] 朱双慧 李珠银 丁文珺 LIU Yuehua;YANG Zhaoyuan;LU Jicong;XIE Mengxia;GUO Jing;WANG Yuanyuan;YANG Jingjing;TIAN Yun;ZHAO Ming;CHEN Dongsun;ZHU Shuanghui;LI Zhuyin;DING Wenjun(Department of Obstetrics,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052)

机构地区：[1]郑州大学第三附属医院产科,郑州450052

出　　处：《郑州大学学报（医学版）》2024年第3期320-325,共6页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省医学科技攻关项目(SBGJ202002122,LHGJ20190364)。

摘　　要：目的:探讨miR-34c-5p与poFUT1的靶向关系及对胎盘血管形成的影响。方法:利用ENCORI数据库预测miR-34c-5p与poFUT1的结合位点,采用双荧光素酶报告实验验证。随机选取2023年4至10月在郑州大学第三附属医院住院的胎儿生长受限(FGR)孕妇20例(FGR组),选择同期正常孕妇20例为对照。采用实时荧光定量PCR法检测两组胎盘组织中miR-34c-5p和poFUT1 mRNA的表达。取脐静脉内皮细胞,分组转染miR-34c-5p模拟物及其对照(NC)、miR-34c-5p抑制物及其NC、si-poFUT1 NC、si-poFUT1、si-poFUT1+miR-34c-5p抑制物,采用CCK-8法、Transwell实验以及成管实验检测细胞转染24、48、72、96 h后的增殖能力,转染48 h后的侵袭能力和成管能力。结果:FGR组和对照组胎盘组织中miR-34c-5p表达水平分别为(1.57±0.39)、(1.09±0.18),poFUT1 mRNA表达水平分别为(0.51±0.17)、(1.06±0.13),FGR组胎盘组织中miR-34c-5p表达水平高于对照组,poFUT1 mRNA表达水平低于对照组(P<0.05)。与miR-34c-5p模拟物NC组比较,模拟物组侵袭细胞数和管腔节点数减少,细胞增殖活性降低(P<0.05)。与miR-34c-5p抑制物NC组比较,抑制物组侵袭细胞数和管腔节点数增加,细胞增殖活性升高(P<0.05)。与si-poFUT1 NC组相比,si-poFUT1组侵袭细胞数和管腔节点数减少,细胞增殖活性降低(P<0.05),而si-poFUT1+miR-34c-5p抑制物组上述变化较si-poFUT1组部分逆转(P<0.05)。结论:miR-34c-5p可能通过调控poFUT1影响血管内皮细胞功能,从而影响胎盘的血管形成,参与FGR的发生。Aim:To investigate the targeting relationship between miR-34c-5p and poFUT1 and the effcet on placental angiogenesis.Methods:The binding sites between miR-34c-5p and poFUT1 were predicted by ENCORI database and verified by dual luciferase reporter assay.A total of 20 pregnant women with fetal growth restriction(FGR)from the Third Affiliated Hospital of Zhengzhou University from April to October 2023 were randomly selected,with 20 normal pregnant women during the same period serving as control.The expressions of miR-34c-5p and poFUT1 mRNA in placental tissue were detected using quantitative PCR.Human umbilic vein endothelial cells were transfected with miR-34c-5p mimic NC,miR-34c-5p mimic,miR-34c-5p inhibitor NC,miR-34c-5p inhibitor,si-poFUT1 NC,si-poFUT1,si-poFUT1+miR-34c-5p inhibitor,respectively,and cell proliferation,invasion and tube formation were verified using the CCK-8 assay,transwell assay,and tube formation experiment.Results:The expression of miR-34c-5p was(1.57±0.39),(1.09±0.18),and that of poFUT1 mRNA was(0.51±0.17),(1.06±0.13)in FGR and normal placental tissue;compared with control group,the expression of miR-34c-5p of FGR group was higher,and that of poFUT1 mRNA was lower(P<0.05).The number of invasive cells in miR-34c-5p mimic group was less,and tube formation capacity and the cell proliferative activity were lower than those of mimic NC group(P<0.05).The number of invasive cells in miR-34c-5p inhibitor group was more,and tube formation capacity and the cell proliferative activity were higher than those of inhibitor NC group(P<0.05).Compared with si-poFUT1 NC group,the number of invasive cells in si-poFUT1 group was less,and tube formation capacity and the cell proliferative activity were lower(P<0.05),while the changes mentioned above in si-poFUT1+miR-34c-5p inhibitor group were reversed partly(P<0.05).Conclusion:MiR-34c-5p could regulate vascular endothelial cell function by poFUT1,thereby affecting placenta angiogenesis,and involve in FGR generation.

关 键 词：miR-34c-5p 胎儿生长受限 poFUT1 血管形成 

分 类 号：R714.2[医药卫生—妇产科学]