Maslinic acid supplementation prevents di(2-ethylhexyl)phthalate-induced apoptosis via PRDX6 in peritubular myoid cells of Chinese forest musk deer  

作　　者：Heran Cao Zhenpeng Li Tianqi Jin Shuyang He Shujuan Liu Long Li Yang Wang Ye Gong Gang Wang Fangxia Yang Wuzi Dong 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University,Shaanxi 712100,China [2]College of Forestry,Northwest A&F University,Shaanxi 712100,China [3]Biology Research Centre of Qin-Mountains Wildlife,Northwest A&F University,Shaanxi 712100,China [4]Shaanxi Qiyuan-Times Agri-Tech Development Co.Ltd.,Shaanxi 725000,China

出　　处：《Journal of Environmental Sciences》2024年第9期47-59,共13页环境科学学报（英文版）

基　　金：supported by the Fund of Sci-Tech Innovation Program of Shaanxi Academy of Forestry(No.SXLK2021-0219);the Science and Technology Project of Shaanxi Province(No.2022SF-512);the Science and Technology Innovation and Achievement Transformation Project of Experimental Demonstration Station(base)of Northwest A&F University(No.TGZX2021-32)。

摘　　要：Chinese forest musk deer(FMD),an endangered species,have exhibited low reproductive rates even in captivity due to stress conditions.Investigation revealed the presence of di(2-ethylhexyl)phthalate(DEHP),an environmental endocrine disruptor,in the serum and skin of captive FMDs.Feeding FMDs with maslinic acid(MA)has been observed to alleviate the stress response and improve reproductive rates,although the precise molecular mechanisms remain unclear.Therefore,this study aims to investigate the molecular mechanisms underlying the alleviation of DEHP-induced oxidative stress and cell apoptosis in primary peritubular myoid cells(PMCs)through MA intake.Primary PMCs were isolated and exposed to DEHP in vitro.The results demonstrated that DEHP significantly suppressed antioxidant levels and promoted cell apoptosis in primary PMCs.Moreover,interfering with the expression of PRDX6 was found to induce excessive reactive oxygen species(ROS)production and cell apoptosis in primary PMCs.Supplementation with MA significantly upregulated the expression of PRDX6,thereby attenuating DEHP-induced oxidative stress and cell apoptosis in primary PMCs.These findings provide a theoretical foundation for mitigating stress levels and enhancing reproductive capacity of in captive FMDs.

关 键 词：Maslinic acid Forest musk deer Di(2-ethylhexyl)phthalate Peritubular myoid cells PRDX6 

分 类 号：X503[环境科学与工程—环境工程]