Enhanced direct gaseous CO_(2) fixation into higher bio-succinic acid production and selectivity  

作　　者：Ziyi Yang Wanling Wu Qing Zhao Irini Angelidaki Samuel Gyebi Arhin Dongliang Hua Yuxiao Zhao Hangyu Sun Guangqing Liu Wen Wang 

机构地区：[1]Biomass Energy and Environmental Engineering Research Center,Beijing University of Chemical Technology,Beijing 100029,China [2]College of Chemical Engineering,Beijing University of Chemical Technology,Beijing 100029,China [3]Department of Chemical and Biochemical Environmental Engineering,Technical University of Denmark,DK-2800,Kgs Lyngby,Denmark [4]Energy Research Institute,Qilu University of Technology(Shandong Academy of Sciences),Shandong Provincial Key Laboratory of Biomass Gasification Technology,Jinan 250014,China

出　　处：《Journal of Environmental Sciences》2024年第9期164-175,共12页环境科学学报（英文版）

基　　金：supported by the Beijing Nova Program(No.Z201100006820022);the National Natural Science Foundation of China(Nos.52100023,U20B2022);the China Postdoctoral Science Foundation(No.2020M680314);the Key Science and Technology Development Program of Xinjiang Corps(No.2021DB006);the EU ACT Coo CE ERA-NET program cofunded with EUDP-2021-Ⅱ(No.64021-2006)。

摘　　要：Utilizing CO_(2)for bio-succinic acid production is an attractive approach to achieve carbon capture and recycling(CCR)with simultaneous production of a useful platform chemical.Actinobacillus succinogenes and Basfia succiniciproducens were selected and investigated as microbial catalysts.Firstly,the type and concentration of inorganic carbon concentration and glucose concentration were evaluated.6 g C/L MgCO_(3)and 24 g C/L glucose were found to be the optimal basic operational conditions,with succinic acid production and carbon yield of over 30 g/L and over 40%,respectively.Then,for maximum gaseous CO_(2)fixation,carbonate was replaced with CO_(2)at different ratios.The“less carbonate more CO_(2)”condition of the inorganic carbon source was set as carbonate:CO_(2)=1:9(based on the mass of carbon).This condition presented the highest availability of CO_(2)by well-balanced chemical reaction equilibrium and phase equilibrium,showing the best performance with regarding CO_(2)fixation(about 15 mg C/(L·hr)),with suppressed lactic acid accumulation.According to key enzymes analysis,the ratio of phosphoenolpyruvate carboxykinase to lactic dehydrogenase was enhanced at high ratios of gaseous CO_(2),which could promote glucose conversion through the succinic acid path.To further increase gaseous CO_(2)fixation and succinic acid production and selectivity,stepwise CO_(2)addition was evaluated.50%-65%increase in inorganic carbon utilization was obtained coupled with 20%-30%increase in succinic acid selectivity.This was due to the promotion of the succinic acid branch of the glucose metabolism,while suppressing the pyruvate branch,along with the inhibition on the conversion from glucose to lactic acid.

关 键 词：Succinic acid CO_(2) fixation Actinobacillus succinogenes Basfia succiniciproducens Lactic acid 

分 类 号：TQ426.97[化学工程] X701[环境科学与工程—环境工程]