Enhanced organic degradation and microbial community cooperation by inoculating Bacillus licheniformis in low temperature composting  

作　　者：Jing Su Kaiyun Zhou Wenjie Chen Shaoqi Xu Ziwei Feng Yuan Chang Xiaoyan Ding Yi Zheng Xingling Tao Ake Zhang Yue Wang Ji Li Guochun Ding Yuquan Wei 

机构地区：[1]Nanjing Institute of Environmental Sciences,Ministry of Ecology and Environment,Nanjing 210042,China [2]College of Resources and Environmental Science,Beijing Key Laboratory of Biodiversity and Organic Farming,China Agricultural University,Beijing 100193,China [3]Organic Recycling Institute(Suzhou)of China Agricultural University,Wuzhong District,Suzhou 215128,China [4]Fuyang Academy of Agricultural Sciences,Fuyang 236065,China

出　　处：《Journal of Environmental Sciences》2024年第9期189-200,共12页环境科学学报（英文版）

基　　金：supported by the Open Research Fund Program of State Environmental Protection Key Laboratory of Food Chain Pollution Control(No.FC2022YB01);the National Natural Science Foundation of China(No.32071552);the Independent Research Project of Science and Technology Innovation Base in Tibet Autonomous Region(No.XZ2022JR0007G);China Agricultural University-Dabeinong Group Professional degree graduate joint training reform project(No.CAUDBN PDG-JTRP).

摘　　要：Microbial activity and interaction are the important driving factors in the start-up phase of food waste composting at low temperature.The aim of this study was to explore the effect of inoculating Bacillus licheniformis on the degradation of organic components and the potential microbe-driven mechanism from the aspects of organic matter degradation,enzyme activity,microbial community interaction,and microbial metabolic function.The results showed that after inoculating B.licheniformis,temperature increased to 47.8℃ on day2,and the degradation of readily degraded carbohydrates(RDC)increased by 31.2%,and the bioheat production increased by 16.5%.There was an obvious enhancement of extracellular enzymes activities after inoculation,especially amylase activity,which increased by 7.68 times on day 4.The inoculated B.licheniformis colonized in composting as key genus in the start-up phase.Modular network analysis and Mantel test indicated that inoculation drove the cooperation between microbial network modules who were responsible for various organic components(RDC,lipid,protein,and lignocellulose)degradation in the start-up phase.Metabolic function prediction suggested that carbohydrate metabolisms including starch and sucrose metabolism,glycolysis/gluconeogenesis,pyruvate metabolism,etc.,were improved by increasing the abundance of related functional genes after inoculation.In conclusion,inoculating B.licheniformis accelerated organic degradation by driving the cooperation between microbial network modules and enhancing microbial metabolism in the start-up phase of composting.

关 键 词：Microbial agent Organic matter degradation Microbial interaction Food waste Low temperature composting 

分 类 号：X799.3[环境科学与工程—环境工程] X172[农业科学—肥料学] S141.4[农业科学—农业基础科学]